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Discussions on how to propagate your plants sexually and asexually, by seed, natural division or leaf pulling

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By isaacsbees
Posts:  83
Joined:  Sun Feb 07, 2016 8:13 pm
#377902
Alright gang, I want to share a method to germinate and sprout sundew seeds consistently in two weeks or less. Here's the method:

Step 1: Put seeds (around 5-15 micrograms of seed works for me) into a microcentrifuge tube and added distilled, RO, or rainwater to fill the tube.

Step 2: Stratify for 1 week in the fridge with no light. Every day, remove the tube(s) from the fridge and give a vigorous shake (I find that this is an important step).

Step 3: Remove the tube from the fridge, shake it up, and using a pipet, quickly suck up the seed solution and drop the seeds onto wet peat moss. Keep in a bag for high humidity, and under full sun (or LED) @ ~80F for a week, and you should see loads of tiny green shoots.

I know that we've all got the techniques that work for us, just thought I'd share what I do :)

-Isaac


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By isaacsbees
Posts:  83
Joined:  Sun Feb 07, 2016 8:13 pm
#377945
murrkywaters wrote:I'm guessing this doesn't work well with tuberos sundews

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Sorry, I shouldn't have used the umbrella term "sundew." Definitely works best with the non-tuberous varieties :)
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By isaacsbees
Posts:  83
Joined:  Sun Feb 07, 2016 8:13 pm
#377947
Panman wrote:I've had mixed results with this method so I don't know if it is the method or the seeds.
I've tried it on seeds as old as 2 yrs but of course fertility isn't only linked to age. I'm sure you've had more experience with germination than me, being that you run the seed bank :)
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By isaacsbees
Posts:  83
Joined:  Sun Feb 07, 2016 8:13 pm
#377948
Panman wrote:I've had mixed results with this method so I don't know if it is the method or the seeds.
Never mind about the seed bank, haha. Your avatar is the same as ChefDean's so ya fooled me. Still, highly likely you've got more germination experience :)
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By isaacsbees
Posts:  83
Joined:  Sun Feb 07, 2016 8:13 pm
#377954
sundewd wrote:Have you done a comparison of this method vs without the soaking period?
Yes I have, and I've found that the soaking and agitating makes the seed coat more pliable. I'm an undergrad bio major so I have access to lots of awesome microscopes :) In fact, this discovery is a part of a paper I'm writing on CPs, maybe I'll put it up on the forum once it's done.
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By sundewd
Posts:  37
Joined:  Sat Jan 16, 2021 8:53 am
#377958
I might as well try an experiment on some burmannii seeds. What about doing the soak, and forgoing the bag; Do you think that would also increase germination? Do you have an opinion of which step is more beneficial? And which species have you tried this on?
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By murrkywaters
Posts:  590
Joined:  Wed Sep 02, 2020 2:39 pm
#377959
I also have a microscope so I'd be willing to share my observations for your research. Unfortunately mine is like the bottom tier compound optical scope. I really need to get better objectives

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By Apollyon
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Posts:  1118
Joined:  Tue May 05, 2020 2:49 am
#377960
Seems like a good idea, I happen to have a second test tube rack I can do this with and a small fridge. I know Shadowtski is partial to this method. I remember reading him suggesting it to a number of people. I'd love to get a lock on my perlata or grantsaui lol.
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By ChefDean
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Posts:  3110
Joined:  Tue Sep 18, 2018 12:44 am
#377963
isaacsbees wrote: Mon Apr 12, 2021 7:21 pm
Panman wrote:I've had mixed results with this method so I don't know if it is the method or the seeds.
I've tried it on seeds as old as 2 yrs but of course fertility isn't only linked to age. I'm sure you've had more experience with germination than me, being that you run the seed bank :)
In reality, my experience with germination is pretty poor. Seeing as I'm a Bio major too, that's pretty sad.
Supercazzola liked this

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