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Discussions on how to propagate your plants sexually and asexually, by seed, natural division or leaf pulling

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By isaacsbees
Posts:  83
Joined:  Sun Feb 07, 2016 8:13 pm
#377973
murrkywaters wrote:How do you usually sterilize the centrifuge tubes?

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I don't sterilize them, just because I have access to pre-sterilized tubes. If you were to reuse tubes, I think hot soap and water would be sufficient (unless you have an autoclave). Honestly, I don't think sterilization should be too much of an issue :)
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By murrkywaters
Posts:  598
Joined:  Wed Sep 02, 2020 2:39 pm
#377977
Fair enough. They're cheap enough I could always but more. I just don't like waste. No autoclave yet. Once I live somewhere else where I can start doing fungal tissue culture again I'll get one for sure.

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By isaacsbees
Posts:  83
Joined:  Sun Feb 07, 2016 8:13 pm
#377978
murrkywaters wrote:I also have a microscope so I'd be willing to share my observations for your research. Unfortunately mine is like the bottom tier compound optical scope. I really need to get better objectives

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If you decide to do the method let me know your results :)
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By Panman
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Posts:  1505
Joined:  Wed Mar 04, 2020 8:41 pm
#377979
isaacsbees wrote: Mon Apr 12, 2021 7:23 pmNever mind about the seed bank, haha. Your avatar is the same as ChefDean's so ya fooled me.
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By murrkywaters
Posts:  598
Joined:  Wed Sep 02, 2020 2:39 pm
#378000
isaacsbees wrote:
murrkywaters wrote:I also have a microscope so I'd be willing to share my observations for your research. Unfortunately mine is like the bottom tier compound optical scope. I really need to get better objectives

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If you decide to do the method let me know your results :)
I've got a handful of species that I could try this on

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By murrkywaters
Posts:  598
Joined:  Wed Sep 02, 2020 2:39 pm
#379773
I had a hard time putting seeds on slides without losing them. I should probably get a crystalizing dish. The tubes were just too opaque. Week one is done tomorrow.

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By MaxVft
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Posts:  58
Joined:  Sat May 08, 2021 4:17 am
#381245
Are there any alternatives to microcentifruge tubes?'Cuz I can just use a plastic container for the seeds.
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By MaxVft
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Posts:  58
Joined:  Sat May 08, 2021 4:17 am
#381248
Ok,I got some Drosera spatulata seeds coming in the mail from the seed bank,so I guess I'll try this method out.
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By murrkywaters
Posts:  598
Joined:  Wed Sep 02, 2020 2:39 pm
#381249
The only species that has 0 germination is the ultrimafica, the rest have the saturated GA3 at the highest rate and the water close behind. Nothing from any of the 10ppm maxsea groups

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By murrkywaters
Posts:  598
Joined:  Wed Sep 02, 2020 2:39 pm
#381251
MaxVft wrote:Ok,I got some Drosera spatulata seeds coming in the mail from the seed bank,so I guess I'll try this method out.
Try it with only part of the seed pack. It's best to save some seeds as backup.

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