Tissue Culture FAQ

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lemonlily

 
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Re: Tissue Culture FAQ

by lemonlily » Thu Oct 15, 2009 3:48 pm

Jello? Mmm... The stuff to make the soil stuff? That jelly stuff the plant lives on?
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hackerberry
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Re: Tissue Culture FAQ

by hackerberry » Thu Oct 15, 2009 7:14 pm

gage wrote:alright, do you know if they raise or lower the ph very much?


It raises the ph a little, jellos are more towards the alkaline side.

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Re: Tissue Culture FAQ

by gage » Thu Oct 15, 2009 8:46 pm

ok so i shouldnt need to factor that into the ph should i?

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Matt
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Re: Tissue Culture FAQ

by Matt » Thu Oct 15, 2009 9:18 pm

Not really. Maybe make the pH slightly more acidic (0.1 or so) to compensate.
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Re: Tissue Culture FAQ

by gage » Thu Oct 15, 2009 9:38 pm

okay alright and how much to add per liter?

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Re: Tissue Culture FAQ

by goldslinger » Mon Oct 26, 2009 9:27 pm

Gage,

That You will have to experiment with and tell Us!

If You want to add to each jar individually before autoclaving it, I would make a dry run batch with just sugar, water to save on supplies; then adjust PH, then just add say a level smidgen to 25ml in a baby food jar, boil in microwave; (to simulate pressure cooking) cool and see how it sets up at room temp. If it collapses when You tip it, or too liquid, try a slight heaping smidgen, etc. until You have it, I guess.

That's kinda what I did.

Good luck!

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Re: Tissue Culture FAQ

by PitcherPlanter » Fri Dec 25, 2009 8:00 pm

What is BAP?

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Matt
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Re: Tissue Culture FAQ

by Matt » Fri Dec 25, 2009 8:16 pm

PitcherPlanter wrote:What is BAP?

BAP is a plant growth regulator of the cytokinin family also known as 6-BENZYLAMINOPURINE (BA):
http://en.wikipedia.org/wiki/Benzylaminopurine
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Re: Tissue Culture FAQ

by goldslinger » Fri Dec 25, 2009 11:06 pm

hackerberry wrote:
gage wrote:alright, do you know if they raise or lower the ph very much?


It raises the ph a little, jellos are more towards the alkaline side.

hb


So does Gelcarin, ( http://www.phytotechlab.com/Detail.aspx?ID=200 ) I've come to find out. Raises the PH by about
.6 or .7 in the batches I've done.
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Matt
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Re: Tissue Culture FAQ

by Matt » Sat Dec 26, 2009 1:52 am

goldslinger wrote:So does Gelcarin, ( http://www.phytotechlab.com/Detail.aspx?ID=200 ) I've come to find out. Raises the PH by about
.6 or .7 in the batches I've done.

Oh, thanks for letting us know that Gary! I haven't tested pH after adding Gelcarin, so it's good to know.
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Re: Tissue Culture FAQ

by goldslinger » Thu Dec 31, 2009 2:05 am

Matt,

How are Your cultures doing on the Gelcarin at 7 Grams/Liter so far?

Gary
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Re: Tissue Culture FAQ

by Matt » Thu Dec 31, 2009 5:14 am

Hey Gary,
They appear to be doing about the same as the agar. I can't really tell a difference. I like how the media is clearer, but honestly I can't really tell much of any difference in agar vs gelcarin. I'm using it at the same concentration as agar. I don't measure how many grams per liter, but rather dispense it individually into each baby food jar. I use one level (or heaping) "pinch" spoon per 35 to 40ml of media. It seems to solidify about the same as agar, maybe a bit firmer, but definitely usable still.

Maybe in another 2 to 3 weeks I can really report the difference. Right now the cultures are just hitting their stride and rapidly growing. In another 3 weeks, they will have nearly filled the jars, or at least should have.
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Re: Tissue Culture FAQ

by Xeno » Tue Jan 05, 2010 12:24 am

Matt you got many diff vfs invitro from explants now ?

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Re: Tissue Culture FAQ

by Matt » Tue Jan 05, 2010 3:43 am

Xeno wrote:Matt you got many diff vfs invitro from explants now ?

Yeah, I've got about a dozen different clones going now.
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Re: Tissue Culture FAQ

by Xeno » Tue Jan 05, 2010 1:24 pm

Very nice Matt since it wasnt that long since you did start from what I can recall. I just hope that I can get some luck
to after I have trained some on seeds. Btw Iff I manage to invitro for example Tiger teeth or such when I am done multiplaying
that clone. Its then possible to have one in a jarr for a long time for later use and then I could use that one for explant since its
already clean ?


Matt wrote:
Xeno wrote:Matt you got many diff vfs invitro from explants now ?

Yeah, I've got about a dozen different clones going now.

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