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By lemonlily
Posts:  3167
Joined:  Thu Oct 16, 2008 10:54 pm
#29591
Jello? Mmm... The stuff to make the soil stuff? That jelly stuff the plant lives on?
By hackerberry
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Posts:  1704
Joined:  Fri Feb 13, 2009 7:58 pm
#29619
gage wrote:alright, do you know if they raise or lower the ph very much?
It raises the ph a little, jellos are more towards the alkaline side.

hb
By gage
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Posts:  170
Joined:  Thu Sep 17, 2009 8:23 pm
#29622
ok so i shouldnt need to factor that into the ph should i?
By gage
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Posts:  170
Joined:  Thu Sep 17, 2009 8:23 pm
#29630
okay alright and how much to add per liter?
By goldslinger
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Posts:  772
Joined:  Thu Jul 09, 2009 1:31 am
#31159
Gage,

That You will have to experiment with and tell Us!

If You want to add to each jar individually before autoclaving it, I would make a dry run batch with just sugar, water to save on supplies; then adjust PH, then just add say a level smidgen to 25ml in a baby food jar, boil in microwave; (to simulate pressure cooking) cool and see how it sets up at room temp. If it collapses when You tip it, or too liquid, try a slight heaping smidgen, etc. until You have it, I guess.

That's kinda what I did.

Good luck!

Gary
By goldslinger
Location: 
Posts:  772
Joined:  Thu Jul 09, 2009 1:31 am
#36547
hackerberry wrote:
gage wrote:alright, do you know if they raise or lower the ph very much?
It raises the ph a little, jellos are more towards the alkaline side.

hb
So does Gelcarin, ( http://www.phytotechlab.com/Detail.aspx?ID=200 ) I've come to find out. Raises the PH by about
.6 or .7 in the batches I've done.
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By Matt
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Posts:  22325
Joined:  Mon Apr 21, 2008 11:28 pm
#36572
goldslinger wrote:So does Gelcarin, ( http://www.phytotechlab.com/Detail.aspx?ID=200 ) I've come to find out. Raises the PH by about
.6 or .7 in the batches I've done.
Oh, thanks for letting us know that Gary! I haven't tested pH after adding Gelcarin, so it's good to know.
By goldslinger
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Posts:  772
Joined:  Thu Jul 09, 2009 1:31 am
#37482
Matt,

How are Your cultures doing on the Gelcarin at 7 Grams/Liter so far?

Gary
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By Matt
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Posts:  22325
Joined:  Mon Apr 21, 2008 11:28 pm
#37508
Hey Gary,
They appear to be doing about the same as the agar. I can't really tell a difference. I like how the media is clearer, but honestly I can't really tell much of any difference in agar vs gelcarin. I'm using it at the same concentration as agar. I don't measure how many grams per liter, but rather dispense it individually into each baby food jar. I use one level (or heaping) "pinch" spoon per 35 to 40ml of media. It seems to solidify about the same as agar, maybe a bit firmer, but definitely usable still.

Maybe in another 2 to 3 weeks I can really report the difference. Right now the cultures are just hitting their stride and rapidly growing. In another 3 weeks, they will have nearly filled the jars, or at least should have.
By Xeno
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Posts:  524
Joined:  Wed Sep 16, 2009 2:10 pm
#38286
Matt you got many diff vfs invitro from explants now ?
By Xeno
Location: 
Posts:  524
Joined:  Wed Sep 16, 2009 2:10 pm
#38346
Very nice Matt since it wasnt that long since you did start from what I can recall. I just hope that I can get some luck
to after I have trained some on seeds. Btw Iff I manage to invitro for example Tiger teeth or such when I am done multiplaying
that clone. Its then possible to have one in a jarr for a long time for later use and then I could use that one for explant since its
already clean ?

Matt wrote:
Xeno wrote:Matt you got many diff vfs invitro from explants now ?
Yeah, I've got about a dozen different clones going now.
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