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Sterilizing

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Sterilizing

By rpe
Posts:  103
Joined:  Thu Nov 13, 2008 7:59 pm
-  Wed Feb 16, 2022 1:50 am #400324
Do you presterilize your containers before adding media?
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Re: Sterilizing

By MikeB
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Posts:  1919
Joined:  Sat Apr 25, 2020 4:13 pm
-  Wed Feb 16, 2022 2:29 am #400327
Manually? No. I rinse out pots and let them sit in the sun for at least a week before reuse so the sun's UV rays can take care of that.

Re: Sterilizing

By rpe
Posts:  103
Joined:  Thu Nov 13, 2008 7:59 pm
-  Wed Feb 16, 2022 2:45 am #400331
Yes I do mean the baby food containers or whatever you are using for TC. Thanks Panman for pointing that out.
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Re: Sterilizing

By MikeB
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Posts:  1919
Joined:  Sat Apr 25, 2020 4:13 pm
-  Wed Feb 16, 2022 3:21 am #400335
Again, I need to read the forum title before posting a reply.... :oops:

Yes, you want to sterilize the TC container before use. Otherwise, bacteria and/or mold will wreak havoc on your plants and media.
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Re: Sterilizing

By Hendre
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Posts:  91
Joined:  Sun Mar 29, 2020 2:03 pm
-  Wed Feb 16, 2022 8:55 am #400343
Always sterilise just about everything going into tissue culture. Here at uni I use the autoclave @121c for 20 minutes. At home you can use a pressure cooker to do so, just don't seal your containers entirely or they may explode!
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