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By avadakedavra
Posts:  90
Joined:  Thu May 18, 2017 2:06 pm
#308783
I got some gemmae of Drosera.
I want to start these by Tissue Culture.

But I cannot find how I sterilize Gemmae on web sites.

Can I sterilize it by bleach (I used to use for seeds) ?
Or should I use mild sterilization media like weak hydrogenperoxcide ?

Please give me some information or your idea.

I get four kinds of pygmy drosera and 5 gemmae for each.



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By avadakedavra
Posts:  90
Joined:  Thu May 18, 2017 2:06 pm
#308878
I found a post about TC of Drosera from gemmae.
won0507won wrote:
plantmanmax wrote:How did you sterilize the gemmae?
Um.. ( I can't remember well,) I did;

A: 5min in approximately 1~2mg of benzimidazole in approx. 40ml of sterilized water, and 5min 10% bleach. (and rinse, of course.)
B: 10min 10% bleach and rinse.

Both aorked well, but method B could be kill some gemmae. About 10~20% of gemmaes are killed by method B.

Please understand my English ability :)

I will do like this.


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By Tobi K
Posts:  66
Joined:  Thu Jan 06, 2011 9:30 am
#310791
pygmy drosera have very sensitive roots. How can it be potted to acclimate in peat without damage it?
By TissueBob
Posts:  8
Joined:  Sun Jan 07, 2018 7:43 pm
#311770
Nice one! How do you go to propagate them? Only growing pygmy drosera in vitro doenst make sense to me. Or was it a "just for fun" trial?
User avatar
By avadakedavra
Posts:  90
Joined:  Thu May 18, 2017 2:06 pm
#317034
Tobi K wrote:pygmy drosera have very sensitive roots. How can it be potted to acclimate in peat without damage it?
I’m not sure but they prefer soft medium for their root.
When I replate them just put the plant on TC medium.


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By avadakedavra
Posts:  90
Joined:  Thu May 18, 2017 2:06 pm
#317035
TissueBob wrote:Nice one! How do you go to propagate them? Only growing pygmy drosera in vitro doenst make sense to me. Or was it a "just for fun" trial?
both. I enjoy this trial.
I wanna try to keep them healthy in vitro, and then propagate them if I can.
some of them made callus on hormone free medium.Image

some of them died but I have no idea cuz same gammae and same medium and same place I kept, I found both fine and bad.

Image


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