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By anansi
Posts:  8
Joined:  Fri Dec 27, 2019 10:02 am
#358544
Hi everyone, I'm about to try tissue culture. I've read the FAQs thread about it and it's been very helpful. Just have one stupid question: how do I prepare a Cytokinin solution? I know that it should be 1g/L but what does liter mean? Is it simply 1L of distilled water? Or is it more complicated than that?

What I have is BAP.

Looking forward to your help.

Thanks

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By Matt
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Posts:  22479
Joined:  Mon Apr 21, 2008 11:28 pm
#358552
anansi wrote: I know that it should be 1g/L but what does liter mean? Is it simply 1L of distilled water? Or is it more complicated than that?
Nope, not more complicated than that. You have it right. Just mix the 1g of whatever cytokinin you have into the water and then use it. Of course, not all plant growth regulators are soluble in water, including BAP, so you'll have to use a chemical solvent to get it to mix into the water. BAP is soluble in both NaOH and KOH.

You'll need to do more research as to how to make a solution of one of those solvents to dissolve the BAP powder. Though I've done it before with KOH (like 10 years ago) I can't recall the calculation now and don't bother with a solvent anymore. Instead, I just order the PGRs already in liquid form at a concentration of 1mg/ml. It saves a little time and work.
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