FlytrapCare Carnivorous Plant Forums

Sponsored by FlytrapStore.com

Discuss all micropropagation related topics here.

Moderator: Matt

By anansi
Posts:  8
Joined:  Fri Dec 27, 2019 10:02 am
#358544
Hi everyone, I'm about to try tissue culture. I've read the FAQs thread about it and it's been very helpful. Just have one stupid question: how do I prepare a Cytokinin solution? I know that it should be 1g/L but what does liter mean? Is it simply 1L of distilled water? Or is it more complicated than that?

What I have is BAP.

Looking forward to your help.

Thanks

Sent from my IN2020 using Tapatalk

User avatar
By Matt
Location: 
Posts:  22436
Joined:  Mon Apr 21, 2008 11:28 pm
#358552
anansi wrote: I know that it should be 1g/L but what does liter mean? Is it simply 1L of distilled water? Or is it more complicated than that?
Nope, not more complicated than that. You have it right. Just mix the 1g of whatever cytokinin you have into the water and then use it. Of course, not all plant growth regulators are soluble in water, including BAP, so you'll have to use a chemical solvent to get it to mix into the water. BAP is soluble in both NaOH and KOH.

You'll need to do more research as to how to make a solution of one of those solvents to dissolve the BAP powder. Though I've done it before with KOH (like 10 years ago) I can't recall the calculation now and don't bother with a solvent anymore. Instead, I just order the PGRs already in liquid form at a concentration of 1mg/ml. It saves a little time and work.
A CP dream come true

You guys are all lucky. I can't find any of thes[…]

Ohh I thought only the sarracenias seeds are lev[…]

I agree with MaxVft, the soil contains too much […]

west facing widow works great for me.

SD Kronos

I also think that the size of the plant plays a hu[…]

When the soil goes "sour", flytraps g[…]

Trying different settings

So I have a few plants inside a “terrarium&r[…]

Where do I buy Neps online?

Sarracenia Northwest occasionally has some https:[…]

Support the community - Shop at FlytrapStore.com!