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Tissue culture media turning yellow

Moderator: Matt

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Tissue culture media turning yellow#367972

By PintoVulgaris -  Mon Oct 26, 2020 6:18 pm
Posts:  1
Joined:  Sat Oct 24, 2020 4:06 pm

- Mon Oct 26, 2020 6:18 pm #367972

I used 0.07 grams of MS micro and macronutrients (M153), 1ml PPM, 1ml IBA with a pH of 5.6 for C. follicularis. After two days the media began turning yellow/orange. I'm not sure what this means, is the pH too low?

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Re: Tissue culture media turning yellow#368171

By Matt -  Thu Oct 29, 2020 3:34 pm
Location:  near Festus, Missouri
Posts:  22523
Joined:  Mon Apr 21, 2008 11:28 pm

- Thu Oct 29, 2020 3:34 pm #368171

Usually, that happens when tissue is dead or dying. Any sort of plant growth regulators will speed up that yellowing process of damaged or dying tissue as well.

That Cephalotus looks pretty large compared to most of mine in TC. Did you grow it to that size? If so, what was the media you used?

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