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By bluestatic
Posts:  40
Joined:  Tue Jun 27, 2017 7:32 pm
#299661
A couple years ago I tried tissue culture and was successful with a few of the jars not being contaminated. I forgot the terminology but the nutrients you mix with agar and pour into the bottles - how long does that last for? I have quite a few jars I never used with the mixture in the bottom. None are contaminated. Could I try tissue culturing with those left over from years past or best to just start over.

Also the powder, and chemicals to make the "gel" with I have kept in the refrigerator. Is there a shelf life on those as well?
By TCGator
Posts:  44
Joined:  Fri Jan 16, 2015 9:21 pm
#300098
A premixed powder (I assume that you are talking about the nutrient mix) will last for a long time if kept refrigerated and sealed so that moisture is not sucked into it. Fresh premixed powder will be a cream color. I have used slightly yellowed mix with no issues. If very yellow or cakey, I would not use.

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