Tissue culture seeds

Tissue culture seeds#293199

By avadakedavra -  Fri May 19, 2017 4:42 am
Posts:  90
Joined:  Thu May 18, 2017 2:06 pm

- Fri May 19, 2017 4:42 am #293199

I sowed some seeds of
Dionaea muscipula
Drosera occidentalis
Drosera rotundifolia
Sarracenia minor
Nepenthes albomarguinata
on 1st May 2017

Nepenthes and some Dionaea muscipula are under the light and temp is 20-25 degree Celsius.

Others are in refrigerator for cold term.

Tissue culture medium is 1/2 MS and 20g suger and agar.
And I added toooo much BAP in it !!

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Re: Tissue culture seeds#293200

By avadakedavra -  Fri May 19, 2017 4:50 am
Posts:  90
Joined:  Thu May 18, 2017 2:06 pm

- Fri May 19, 2017 4:50 am #293200

avadakedavra wrote:I sowed some seeds of
Dionaea muscipula
Drosera occidentalis
Drosera rotundifolia
Sarracenia minor
Nepenthes albomarguinata
on 1st May 2017

Nepenthes and some Dionaea muscipula are under the light and temp is 20-25 degree Celsius.

Others are in refrigerator for cold term.

Tissue culture medium is 1/2 MS and 20g suger and agar.
And I added toooo much BAP in it !!

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No seed is germinating yet.
Should I reset the seeds by new medium ?

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Re: Tissue culture seeds#293205

By Smooter80 -  Fri May 19, 2017 5:09 am
Posts:  1038
Joined:  Wed Feb 17, 2016 5:33 pm

- Fri May 19, 2017 5:09 am #293205

25C is a bit low for your max temp. For the VFTs and the
Albo, I would bump that up to 30C. It's too soon to make any changes. Germination for both is common around 1 month.

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Re: Tissue culture seeds#293216

By avadakedavra -  Fri May 19, 2017 2:49 pm
Posts:  90
Joined:  Thu May 18, 2017 2:06 pm

- Fri May 19, 2017 2:49 pm #293216

Smooter80 wrote:25C is a bit low for your max temp. For the VFTs and the
Albo, I would bump that up to 30C. It's too soon to make any changes. Germination for both is common around 1 month.

Thanks !
I will keep over 25 C .
and wait for germination .

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Re: Tissue culture seeds#293219

By xr280xr -  Fri May 19, 2017 3:13 pm
Posts:  2807
Joined:  Wed Jun 22, 2011 3:29 pm

- Fri May 19, 2017 3:13 pm #293219

Yes, 25-30 would be good. Just don't go much hotter than 30. You don't need to change the media unless it is drying out or it becomes contaminated (in which case you are supposed to throw it out.)

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Wanted:
S. leuco "H.C. White"
D. muscipula: "Microdent", "Dentate"
C. follicularis: Hummer's Giant

Re: Tissue culture seeds#293220

By avadakedavra -  Fri May 19, 2017 3:26 pm
Posts:  90
Joined:  Thu May 18, 2017 2:06 pm

- Fri May 19, 2017 3:26 pm #293220

xr280xr wrote:Yes, 25-30 would be good. Just don't go much hotter than 30. You don't need to change the media unless it is drying out or it becomes contaminated (in which case you are supposed to throw it out.)

I always give up when contamination occurs.
If contamination is in only medium, I pick seed or sprout up and put them on clean soil.

My first TC experience was cacti seeds.

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