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Discuss all micropropagation related topics here.

Moderator: Matt

By David F
Posts:  1649
Joined:  Sun Jan 02, 2011 8:41 pm
#286896
BigBella wrote:
Matt wrote:
blucksy wrote:Thanks! Flaming sounds a little extreme. Could I light a candle and just flame that way?
Even the best of flames don't get hot enough to kill all bacteria. A combination of alcohol and flame does a decent job, but bacteria containation is still common.

Best to run the tools through a pressure cooker and then alcohol dip and flame them between touching anything with them.
I should have mentioned that I store my tools, while working, in a small beaker of 99% isopropyl alcohol and flame them between each use; either that, it use a glass bead sterilizer, but those are generally not used by those just outfitting their labs . . .

I think beads are the way to go in this situation. Particularly since they don't produce air turbidity the way an alcohol or bunsen burner might, especially in a confined space without proper air filtration/ in a home. There are some good compact units out there that aren't too expensive. I don't know if a less expensive bead unit counts as half-assing it, since I'm assuming there's not any HEPA filtration going on.

I probably wouldn't go through the trouble of doing TC without at least a box, but on the same note I'd much prefer aseptic flowing air.
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