- Thu Jul 07, 2011 3:15 am
#111259
Today for the first time i did TC, something i have been posponing for so long, and even today i thought about it. But i think it was worth for the first time.
I started at 3pm and finished at about 8pm, so it took me more than 4 hours to finish. Whether or not if i did it correctly time will tell.
I was going to write everything i did, but honestly with all the things you need to do at once, it's very hard to write it out, or even explain it, i'm saturated with all the time it took, so everything is mix in my head. Matt- now i understand you more than ever, so those people that ask matt when are they're going to be available (including me), do TC, and then talk to Matt about it hehehe!
Well sounds like i did everything right, but the time will tell hopefully!
One of the biggest mistakes i did was:
I put seeds first in 10% bleach rather than 91% ethanol alcohol, so i had to repeat everything again.
Probably the most difficult part was cleaning the tissue, one of the things i did 3 times and forgot about, was when putting the tisue from the sterile water to the media, i forgot to rinse the tweezers 3 times, what i did was: keep the tweezers in 91% ethanol, some sterile water, another sterile water for the tissues, so i did from alcohol to sterile to sterile water pick tissue and put in media then alcohol for tweezers again......and so on, but i forgot to do it 3 times with 3 tissues i don't even remember what they were. So i pick tissue put in media, pick tissue put in media, pick tissue put in media, that was a terrible mistake, but they might work out!
Well wish me luck!
Here's some pics:
I used my tank as my working area, the plastic was to protect even more from particles. Something i might do next time close the tank or aquarium completely with plastic, and make just two holes for the hands, and i'm able to keep everything more sterile!
Oh and yes, i cleaned the kitchen with bleach the night before, and also before starting, all the doors i close, and everything.
Pressure cooker:
Some materials:
I labeled everything:
Everything in their own bag:
labeled more :
Some vessels out of the pressure cooker:
I sprayed 50% alcohol inside the working area:
I'm keeping everything by a #, since i decided to start backwards i started with 86, the reason i'm keeping it with #
s is because i have created a table in excel in order of: #, name of the tissue, date put in TC, days in culture, swelling, shoots or roots, callus, contamination, other observations. and it also keeps everything more organize with just a single number and not the name of the tissue.
Some VFT seeds, the few i got from my plants! there is Czech giant x Akay Ryu, and Dutch X Akay Ryu, a really nice cross i would say, so i do hope the babies grow and germinate!:
Some tissue:
Thanks for watching and i'll be posting here if anything happens.
Thanks for the Dionea for helping me a lot and giving me some advices! Also to matt for the well and wonderful TC FAQ, and all the info about TC. And last but not least, thanks to the knowlegeable members who have answer my questions, and have helped me a lot!
Now is just a matter of waiting, my next TC date will be sometime next week, or this week.
I started at 3pm and finished at about 8pm, so it took me more than 4 hours to finish. Whether or not if i did it correctly time will tell.
I was going to write everything i did, but honestly with all the things you need to do at once, it's very hard to write it out, or even explain it, i'm saturated with all the time it took, so everything is mix in my head. Matt- now i understand you more than ever, so those people that ask matt when are they're going to be available (including me), do TC, and then talk to Matt about it hehehe!
Well sounds like i did everything right, but the time will tell hopefully!
One of the biggest mistakes i did was:
I put seeds first in 10% bleach rather than 91% ethanol alcohol, so i had to repeat everything again.
Probably the most difficult part was cleaning the tissue, one of the things i did 3 times and forgot about, was when putting the tisue from the sterile water to the media, i forgot to rinse the tweezers 3 times, what i did was: keep the tweezers in 91% ethanol, some sterile water, another sterile water for the tissues, so i did from alcohol to sterile to sterile water pick tissue and put in media then alcohol for tweezers again......and so on, but i forgot to do it 3 times with 3 tissues i don't even remember what they were. So i pick tissue put in media, pick tissue put in media, pick tissue put in media, that was a terrible mistake, but they might work out!
Well wish me luck!
Here's some pics:
I used my tank as my working area, the plastic was to protect even more from particles. Something i might do next time close the tank or aquarium completely with plastic, and make just two holes for the hands, and i'm able to keep everything more sterile!
Oh and yes, i cleaned the kitchen with bleach the night before, and also before starting, all the doors i close, and everything.
Pressure cooker:
Some materials:
I labeled everything:
Everything in their own bag:
labeled more :
Some vessels out of the pressure cooker:
I sprayed 50% alcohol inside the working area:
I'm keeping everything by a #, since i decided to start backwards i started with 86, the reason i'm keeping it with #
s is because i have created a table in excel in order of: #, name of the tissue, date put in TC, days in culture, swelling, shoots or roots, callus, contamination, other observations. and it also keeps everything more organize with just a single number and not the name of the tissue.
Some VFT seeds, the few i got from my plants! there is Czech giant x Akay Ryu, and Dutch X Akay Ryu, a really nice cross i would say, so i do hope the babies grow and germinate!:
Some tissue:
Thanks for watching and i'll be posting here if anything happens.
Thanks for the Dionea for helping me a lot and giving me some advices! Also to matt for the well and wonderful TC FAQ, and all the info about TC. And last but not least, thanks to the knowlegeable members who have answer my questions, and have helped me a lot!
Now is just a matter of waiting, my next TC date will be sometime next week, or this week.
Last edited by jht-union on Thu Jul 07, 2011 3:15 pm, edited 1 time in total.
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Looking for these species: http://www.flytrapcare.com/phpBB3/post97520.html