FlytrapCare Carnivorous Plant Forums

I just go this reply from the home tissue org site

I was going to use Bap but now ?

Hi BAP hormone don´t work on VTF.. you must use kinetin.
regards
Victor

BAP will work OK on Dionaea. Just don't use very much. 1mg/L is almost too much. It will cause extreme callus. Kinetin seems to be "gentler" for Dionaea.

i just made my first batch and i used 1ml BAP

Am sure you said on your tissue culture faq to use 1ML BAP ?

I think its going to be a fail because i had my new PH meter in the pan when doing the PH and it slid forward inot the liquid for about 1/2 a second
I tried to continue but am sure the meter is broken i tried to re set it with the bufer solution but it was going abit nuts o well


Here is a list of ingredients for preparing 1 liter of Dionaea muscipula medium:

1/2 MS medium with vitamins (one half of a 1 liter packet or 2.2 grams). Most Tissue Culture Techs uses 1/3 MS for Carnivorous plants with great results.
2 tablespoons of table sugar (about 30g)
1 ml of BAP (1 mg/ml) - leave this out for seeds. It's even optional for explants. Dionaea explants will form callus without it.
1 ml of PPM
Agar
Distilled water
Vinegar and baking soda.

SnapSnapIOM wrote:i just made my first batch and i used 1ml BAP

That's fine.

SnapSnapIOM wrote:Am sure you said on your tissue culture faq to use 1ML BAP ?

Yeah, 1mg/L of BAP will work fine for initializations and multiplications. I used to use that concentration all the time.

So next time maybe use 0.5ml BAP ?

It depends on what you want to achieve. 1mg/L of BAP is fine for initializations and to create a lot of callus to start heavy propagation.

Ugh, not trying to derail but just realized something myself. Matt, what differences would occur when using say 1ml Kinetin versus .5ml Kinetin (1mg/ml) in media to intiate callouses?
Huge enough to be better off throwing the solution away or ok enough to use it and never do it again?

stry67 wrote:Matt, what differences would occur when using say 1ml Kinetin versus .5ml Kinetin (1mg/ml) in media to intiate callouses?

Not much difference really. Kinetin doesn't have as dramatic an effect on Dionaea as BAP does. The difference would be that you'd see a lot more teeny tiny plants with the 1mg/L of Kinetin as compared to the 0.5mg/L once the callus started growing. It would also probably take an extra replate or two to get most of the hormones out of the plants.

stry67 wrote:Huge enough to be better off throwing the solution away or ok enough to use it and never do it again?

No need to throw it away as long as you're OK with taking the extra couple of months to replate the tissue on hormone free media to help get the hormones out of the plants.

Matt wrote:

stry67 wrote:

stry67 wrote:Huge enough to be better off throwing the solution away or ok enough to use it and never do it again?

No need to throw it away as long as you're OK with taking the extra couple of months to replate the tissue on hormone free media to help get the hormones out of the plants.

Yea I was wondering also on a similar thought that activated charcoal in the media might help with that also.

I'm currently using 1ml per liter BAP on a Dente traps. Its callusing like crazy....much more than my last batch of .5ml per liter. I'm trying to get more callus growth so I can separate it and have more dente jars. Idk if i'm going about it wrong...just thought i'd share my limited experience:).

Dionae wrote:I'm currently using 1ml per liter BAP on a Dente traps. Its callusing like crazy....much more than my last batch of .5ml per liter. I'm trying to get more callus growth so I can separate it and have more dente jars. Idk if i'm going about it wrong...just thought i'd share my limited experience:).

Heh, reminds me of a friend. He has a B52 callus that he has kept going for several months now. He cuts out dead parts and removes living sections to start new plants in other vessels.