- Thu Nov 18, 2010 9:11 am
#76236
Hi there!
After many experiments performed using Agar, I decided to switch to Gelrite. With my foodstore agar, I 'managed' to kill some precious drosera species, just because of its chemical variations depending on the batch. The agar I used raised the pH of media solution from desired 5.5 to 7.1 (without me noticing) which - of course- killed many germinating plants. So I got tired of checking the pH of solidified media and re-melting it to sterilize it, and bought 100g of gelrite.
I would like to ask someone who used it before, what is the best concentration and how does it alter pH after addition?
I read that unlike agar, it dissolves in warm water already so the heating stress is lower too.
Thanx for any comments:-)
After many experiments performed using Agar, I decided to switch to Gelrite. With my foodstore agar, I 'managed' to kill some precious drosera species, just because of its chemical variations depending on the batch. The agar I used raised the pH of media solution from desired 5.5 to 7.1 (without me noticing) which - of course- killed many germinating plants. So I got tired of checking the pH of solidified media and re-melting it to sterilize it, and bought 100g of gelrite.
I would like to ask someone who used it before, what is the best concentration and how does it alter pH after addition?
I read that unlike agar, it dissolves in warm water already so the heating stress is lower too.
Thanx for any comments:-)