Sterilizing

Sterilizing#400324

By rpe -  Wed Feb 16, 2022 1:50 am
Posts:  103
Joined:  Thu Nov 13, 2008 7:59 pm

- Wed Feb 16, 2022 1:50 am #400324

Do you presterilize your containers before adding media?

Re: Sterilizing#400327

By MikeB -  Wed Feb 16, 2022 2:29 am
Location:  Clayton, NC, USA - Zone 8a
Posts:  1955
Joined:  Sat Apr 25, 2020 4:13 pm

- Wed Feb 16, 2022 2:29 am #400327

Manually? No. I rinse out pots and let them sit in the sun for at least a week before reuse so the sun's UV rays can take care of that.

Re: Sterilizing#400329

By Panman -  Wed Feb 16, 2022 2:37 am
Location:  Atlanta, GA USA Zone 8a/8b
Posts:  6569
Joined:  Wed Mar 04, 2020 8:41 pm

- Wed Feb 16, 2022 2:37 am #400329

This is in the TC section so I am thinking he means those containers.

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Re: Sterilizing#400331

By rpe -  Wed Feb 16, 2022 2:45 am
Posts:  103
Joined:  Thu Nov 13, 2008 7:59 pm

- Wed Feb 16, 2022 2:45 am #400331

Yes I do mean the baby food containers or whatever you are using for TC. Thanks Panman for pointing that out.

Re: Sterilizing#400335

By MikeB -  Wed Feb 16, 2022 3:21 am
Location:  Clayton, NC, USA - Zone 8a
Posts:  1955
Joined:  Sat Apr 25, 2020 4:13 pm

- Wed Feb 16, 2022 3:21 am #400335

Again, I need to read the forum title before posting a reply....

Yes, you want to sterilize the TC container before use. Otherwise, bacteria and/or mold will wreak havoc on your plants and media.

Re: Sterilizing#400343

By Hendre -  Wed Feb 16, 2022 8:55 am
Location:  South Africa
Posts:  91
Joined:  Sun Mar 29, 2020 2:03 pm

- Wed Feb 16, 2022 8:55 am #400343

Always sterilise just about everything going into tissue culture. Here at uni I use the autoclave @121c for 20 minutes. At home you can use a pressure cooker to do so, just don't seal your containers entirely or they may explode!

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Sterilizing#400324

Re: Sterilizing#400327

Re: Sterilizing#400329

Re: Sterilizing#400331

Re: Sterilizing#400335

Re: Sterilizing#400343

Why the difference in cold stratification?

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