what is tissue culture?

Discuss all micropropagation related topics here.
User Avatar
mitch

 
Posts: 460
Joined: Wed Dec 16, 2009 11:29 pm
Location: sydney, Australia
Thanked: 32 times in 31 posts

what is tissue culture?

by mitch » Sun Jan 03, 2010 7:50 am

hi everyone,

can someone tell me what tissue culture is and how to do it please?

cheers,
mitch
cheers,
mitch

please take a look at my growlist
http://www.flytrapcare.com/phpBB3/mitch-s-growlist-2010-t4681.html

User Avatar
kittyklaws

 
Posts: 1644
Joined: Mon Dec 21, 2009 4:13 am
Location: Bay area, CA
Thanked: 39 times in 38 posts

Re: what is tissue culture?

by kittyklaws » Sun Jan 03, 2010 7:56 am

It's basically taking a piece of the mother plant, like say a leaf cutting, and putting it into a sterile environment within a babyfood jar, and feeding it with this clear jelly nutrient thingy, and it will grow new clones pretty quickly that you can replant. I don't know the specifics though sorry. But good luck! :D And you should definitely look through this thread for some great info: viewtopic.php?f=28&t=1426
Power corrupts, knowledge is power,
study hard, be Evil ;)

User Avatar
mitch

 
Posts: 460
Joined: Wed Dec 16, 2009 11:29 pm
Location: sydney, Australia
Thanked: 32 times in 31 posts

Re: what is tissue culture?

by mitch » Sun Jan 03, 2010 8:32 am

can i do tissue culture without having to buy stuff from every where?
cheers,
mitch

please take a look at my growlist
http://www.flytrapcare.com/phpBB3/mitch-s-growlist-2010-t4681.html

User Avatar
kittyklaws

 
Posts: 1644
Joined: Mon Dec 21, 2009 4:13 am
Location: Bay area, CA
Thanked: 39 times in 38 posts

Re: what is tissue culture?

by kittyklaws » Sun Jan 03, 2010 6:45 pm

umm...you would need that BCP or Kinetin stuff and a bunch of different ingredients:
1/2 MS medium with vitamins (one half of a 1 liter packet or 2.2 grams). Most Tissue Culture Techs uses 1/3 MS for Carnivorous plants with great results.
2 tablespoons of table sugar (about 30g)
1 ml of BAP (1 mg/ml) - leave this out for seeds. It's even optional for explants. Dionaea explants will form callus without it.
1 ml of PPM
Agar
Distilled water
Vinegar and baking soda.
for medium to feed your baby plant, and you need baby food jars and buy these special sealing caps and a pressure cooker.
You should just buy the home tissue culture kit thingy http://www.hometissueculture.org/catalogkits.htm
Power corrupts, knowledge is power,
study hard, be Evil ;)


Return to Tissue Culture

 


  • Related topics
    Replies
    Views
    Last post

Who is online

Users browsing this forum: No registered users and 4 guests