- Wed Dec 02, 2020 6:14 pm
#369868
I'm a complete noob in tissue culture, this is my first try and sadly it failed. My cultures started to turn brown after just 3 days and white mold like contamination. I followed the guide by PhytoTech closely... I used 10% bleach and tween to sterilize my explants for 10 min . Also added ppm to the media. From what I read over the web the browning may be caused by to long time in the sterilization solution. But the white mold I don't seem to understand how it survived the 10 min soak time. They seem contradicting ... Because the long soak time should have killed all the contaminants. Also there is no visible contamination around the rest of the media seems to suggest the transfer was sterile. It's just that the explants were still contaminated. I will try 5 to 6 min soak next to prevent the browning of the explants or maybe some activated charcoal.. but I don't know how to deal with the mold ... I have been googling for days now... hoping for some direction and advice. Hope this forum could give me some insight thank you
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