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Excess moisture

Posted: Fri Dec 07, 2018 1:47 am
by Lorib_nc
Hi all. I'm new to the forum and tissue culture. I've recently gotten my first batch of nepenthes seeds into the culture vessels. 48 hours later there is a pool of fluid on top of the agar. The seeds appear to be floating on the liquid. I can't find anything about excess moisture in any of the texts I've been learning from. Is this something I should be concerned about? Is it too late to do anything about it?

Lori B in NC

Re: Excess moisture

Posted: Sat Dec 08, 2018 12:55 pm
by Eteson
Hi Lori, do not worry too much. This happens sometimes but for me has not been a problem.
How much gelling agent did you used?

Enviado desde mi SM-J700M mediante Tapatalk

Re: Excess moisture

Posted: Mon Dec 10, 2018 11:49 am
by mantis
I let my medium solidify while the jars are slightly tilted.