FlyTrapCare Forums

hi everyone,

can someone tell me what tissue culture is and how to do it please?

cheers,
mitch

It's basically taking a piece of the mother plant, like say a leaf cutting, and putting it into a sterile environment within a babyfood jar, and feeding it with this clear jelly nutrient thingy, and it will grow new clones pretty quickly that you can replant. I don't know the specifics though sorry. But good luck! And you should definitely look through this thread for some great info: http://www.flytrapcare.com/phpBB3/viewt ... =28&t=1426

can i do tissue culture without having to buy stuff from every where?

umm...you would need that BCP or Kinetin stuff and a bunch of different ingredients:
1/2 MS medium with vitamins (one half of a 1 liter packet or 2.2 grams). Most Tissue Culture Techs uses 1/3 MS for Carnivorous plants with great results.
2 tablespoons of table sugar (about 30g)
1 ml of BAP (1 mg/ml) - leave this out for seeds. It's even optional for explants. Dionaea explants will form callus without it.
1 ml of PPM
Agar
Distilled water
Vinegar and baking soda.
for medium to feed your baby plant, and you need baby food jars and buy these special sealing caps and a pressure cooker.
You should just buy the home tissue culture kit thingy http://www.hometissueculture.org/catalogkits.htm