TC contamination
Posted: Tue May 23, 2017 10:35 pm
Hi, l have a question about Tissue culture of Cephalotus follicularis from its leaf.
I've started TC on 19th MAY and now I found some contamination in it.
Something white semitransparent looks like water mold are around some pieces of leaves.
It's not a solid colony of usual mold contamination.
Blank TC mediums are clear and no contamination, so I guess I failed to sterilize the samples of Cephalotus follicularis leaves.
The leaves are still fresh green. They are not dead yet.
May I try re-sterilize and set it again ?
or Keep watching a few days more ?
I have some ethanol, Hypochlorous acid solution and Sodium hypochlorite (as kitchen bleach)
Sent from my iPhone using Tapatalk
I've started TC on 19th MAY and now I found some contamination in it.
Something white semitransparent looks like water mold are around some pieces of leaves.
It's not a solid colony of usual mold contamination.
Blank TC mediums are clear and no contamination, so I guess I failed to sterilize the samples of Cephalotus follicularis leaves.
The leaves are still fresh green. They are not dead yet.
May I try re-sterilize and set it again ?
or Keep watching a few days more ?
I have some ethanol, Hypochlorous acid solution and Sodium hypochlorite (as kitchen bleach)
Sent from my iPhone using Tapatalk