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Moderator: Matt

By floridadancerguy
Posts:  77
Joined:  Sun Jan 17, 2010 10:24 pm
#40126
Does anyone have any Nepenthes seed. I'm looking to start some in TC
Thanks
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By Matt
Location: 
Posts:  21203
Joined:  Mon Apr 21, 2008 11:28 pm
#40239
If you get your hands on some and have luck getting it to germinate, let me know. I've ordered seed from more than 8 different sources and I've yet to have a single Nepenthes seed germinate in vitro or ex vitro. I'm sure that it could be due to getting old seed, but it could also be exposing my ineptitude with Nepenthes seed.
By doku
Location: 
Posts:  346
Joined:  Wed Jul 15, 2009 11:12 pm
#40250
Matt wrote:If you get your hands on some and have luck getting it to germinate, let me know. I've ordered seed from more than 8 different sources and I've yet to have a single Nepenthes seed germinate in vitro or ex vitro. I'm sure that it could be due to getting old seed, but it could also be exposing my ineptitude with Nepenthes seed.
I have bought ventricosa seeds from cascadecarnivores.com and they seem to have a good germinating rate. Around 70% on pure long fiber sphagnum.
By bombsboy
Location: 
Posts:  584
Joined:  Tue Jan 26, 2010 4:57 am
#43509
ive got like 15 n.madagascarensis seeds. ive had them for like a year already so i think they are dead :oops:
dont worry though, i got them for free :D
but i still feel stupid. i tried to germinate them but i failed. :twisted:
By Timmy
Location: 
Posts:  319
Joined:  Sat Jan 16, 2010 4:20 pm
#43542
How do you TC seeds?
By ahicks51
Location: 
Posts:  130
Joined:  Fri Jan 15, 2010 7:13 pm
#44160
Timmy wrote:How do you TC seeds?
Seeds are disinfected (chlorine bleach solution) for 10-25 minutes, washed in sterile distilled water, and placed on sterile plant tissue culture medium. After several weeks to several months, they germinate- provided they didn't get contaminated, and that the seeds are any good, and that the medium you use is a suitable one for them, and that they don't mysteriously die a week or two later for no apparent reason.

Then after a year or two, they're large enough to remove from sterile flask with a decent chance of survival. They don't grow good roots for me in sterile culture, so it's a little like rooting a cutting.
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By MichaelG
Location: 
Posts:  443
Joined:  Thu Sep 17, 2009 2:10 am
#45523
Yes, so they'd be sterile for the tissue culture. I'm pretty sure there are some other solutions, but I'd definitely go with Matt on this one.
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