- Thu Feb 01, 2018 4:23 pm
#310583
Hey guys,
got some problem which appears from time to time. A plant in tissue culture begins to get brownisch and dies off if it not gets replated very soon. If i dont replate this plant dies within one week. If i open the vessel then there is a vacuum an i hear the air going in when pulling up the lid. Then the medium changes the color from clear to yellowish. This appears within hours and at the next day the whole media is yellowiah after contact with fresh O2.
I linked a photo with shows excactley the coloration of plants. For me it is mostly VFF.
Do you have an idea what causes this phenomenon?
kind regards
Bob
got some problem which appears from time to time. A plant in tissue culture begins to get brownisch and dies off if it not gets replated very soon. If i dont replate this plant dies within one week. If i open the vessel then there is a vacuum an i hear the air going in when pulling up the lid. Then the medium changes the color from clear to yellowish. This appears within hours and at the next day the whole media is yellowiah after contact with fresh O2.
I linked a photo with shows excactley the coloration of plants. For me it is mostly VFF.
Do you have an idea what causes this phenomenon?
kind regards
Bob