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Re: Tissue Culture FAQ

Posted: Mon Oct 12, 2009 4:37 am
by gage
yea haha it just seemed like there was something a little more to it

Re: Tissue Culture FAQ

Posted: Mon Oct 12, 2009 4:38 am
by gage
also what other types of media would i use for a tc of a different plant

Re: Tissue Culture FAQ

Posted: Mon Oct 12, 2009 5:17 am
by Matt
gage wrote:also what other types of media would i use for a tc of a different plant
It completely depends on the plant. To my knowledge almost any plant can be grown on one of three types of media:
* Murashige and Skoog
* Knudson C
* Woody Plant
Depending on what kind of plant you want to TC, you'll have to research what kind of media is best.

Re: Tissue Culture FAQ

Posted: Mon Oct 12, 2009 8:41 pm
by gage
okay thanks i will have to do so once i get my tc going

Re: Tissue Culture FAQ

Posted: Tue Oct 13, 2009 12:28 am
by gage
do you need a pressure cooker with a gauge, where would be a cheap place to buy one with a gauge?

Re: Tissue Culture FAQ

Posted: Tue Oct 13, 2009 1:28 am
by Matt
gage wrote:do you need a pressure cooker with a gauge, where would be a cheap place to buy one with a gauge?
I wondered the same thing when I started. No, you don't need one with a gauge. Almost all pressure cookers are set to reach and maintain a pressure of 15 PSI, which is what you want when sterilizing media.

Re: Tissue Culture FAQ

Posted: Tue Oct 13, 2009 1:45 am
by gage
alright thanks, that will save me around 50 bucks

Re: Tissue Culture FAQ

Posted: Tue Oct 13, 2009 4:58 pm
by Aging_Bourbon
Hello ya'll,

well I'm still interested in doing TC and I read the FAQ Sticky but is their other thing's that I should know?



Allen,

Re: Tissue Culture FAQ

Posted: Tue Oct 13, 2009 5:03 pm
by hackerberry
Be Patient hehe. Seriously, there will be tons of questions when you actually start on your TC and not all get the results that everyone else gets. You will develop your own technique as you progress. Like me, I skipped PH testing and scaling. I already created my own method to make things faster to make. I just started using plain Jello instead of agar. Much cheaper but gives you the same result. That's just me.

hb

Re: Tissue Culture FAQ

Posted: Tue Oct 13, 2009 9:17 pm
by gage
hackerberry wrote:Be Patient hehe. Seriously, there will be tons of questions when you actually start on your TC and not all get the results that everyone else gets. You will develop your own technique as you progress. Like me, I skipped PH testing and scaling. I already created my own method to make things faster to make. I just started using plain Jello instead of agar. Much cheaper but gives you the same result. That's just me.

hb

So instead of agar you use gelatin, pectin or jello?

Re: Tissue Culture FAQ

Posted: Tue Oct 13, 2009 9:59 pm
by hackerberry
Yes gage, they do me the same purpose but on the cheaper side.

hb

Re: Tissue Culture FAQ

Posted: Tue Oct 13, 2009 9:59 pm
by Aging_Bourbon
Jello as in Instant jello?

Re: Tissue Culture FAQ

Posted: Tue Oct 13, 2009 10:01 pm
by hackerberry
Jello man....That's what I used in the first place when I was just starting. The sole purpose of Agar is for cell suspension which Jellos do.

hb

Re: Tissue Culture FAQ

Posted: Tue Oct 13, 2009 10:03 pm
by Aging_Bourbon
oh i see now lol.

Re: Tissue Culture FAQ

Posted: Tue Oct 13, 2009 11:31 pm
by gage
alright, do you know if they raise or lower the ph very much?