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By bugman
Posts:  110
Joined:  Mon Jun 13, 2011 1:41 pm
#145044
Has anyone or does anyone propagate sarracenia rhizome or off shoots in tissue culture? I am not I interested in seed, I would like advise or if here is a link to some further info that would be great.
Last edited by bugman on Fri Jun 08, 2012 10:43 pm, edited 1 time in total.
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By Tony C
Posts:  352
Joined:  Wed Mar 07, 2012 10:23 am
#145045
It has been done, but there isn't much info out there. As far as I know all, or nearly all, success has come from commercial labs who aren't too keen to share their methods.
By bugman
Posts:  110
Joined:  Mon Jun 13, 2011 1:41 pm
#145046
Yes info is rather scarce but if there is a will there is a way.

It would be good to know as there are so many hard to get saracenia someone needs to get them in to tissue culture to make them more available
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By Matt
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Posts:  22285
Joined:  Mon Apr 21, 2008 11:28 pm
#145047
bugman wrote:It would be good to know as there are so many hard to get saracenia someone needs to get them in to tissue culture to make them more available
Agreed.

I've done some research, both hands on and investigative, and from what I can gather, flower stalks are the way to go. But I've not yet had one form callus and I've tried all sorts of media mixes and hormone combinations.
By bugman
Posts:  110
Joined:  Mon Jun 13, 2011 1:41 pm
#145049
Have you tried a rhizome pulling similar to the dionaea, pulling of the base if the tube attached to the rhizome?

Or do you attempted to remove a very young off shoot and introduce that?
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By Matt
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Joined:  Mon Apr 21, 2008 11:28 pm
#145091
bugman wrote:Have you tried a rhizome pulling similar to the dionaea, pulling of the base if the tube attached to the rhizome?
I don't use the rhizome on Dionaea because it's too hard to get clean and doesn't form callus very easily. I suspect that the rhizome of a Sarracenia would be extremely hard to get clean as well, so I haven't tried it.
bugman wrote:Or do you attempted to remove a very young off shoot and introduce that?
Nope, I've not tried that, but if I were to make another attempt at starting Sarracenia in TC, I'd probably try newly forming leaves.
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By bugman
Posts:  110
Joined:  Mon Jun 13, 2011 1:41 pm
#145125
Whe you say new leaves are you talking pitcher or phyllodia?
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By Matt
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Posts:  22285
Joined:  Mon Apr 21, 2008 11:28 pm
#145128
bugman wrote:Whe you say new leaves are you talking pitcher or phyllodia?
Both. I don't know what tissue is going to form callus the most easily. In flytraps, the leaves form callus long before flower stalks do. But they're much harder to get clean. I suspect that one of either phyllodia or pitchers will likely form callus more quickly, but I don't have a guess as to which one, nor which hormones to use to induce callus.
By bugman
Posts:  110
Joined:  Mon Jun 13, 2011 1:41 pm
#145129
Well I hope others will add to this thread as it is about time we learnt how to do this
By Stuart
Posts:  3
Joined:  Sun Jun 10, 2012 12:12 pm
#145180
I'm thinking of having a sarracenia that may be worthy of cultivar status tissue cultured next year. If the commercial labs have techniques they're not telling us about, can anyone recommend a European lab for doing it?
By bugman
Posts:  110
Joined:  Mon Jun 13, 2011 1:41 pm
#145234
Well you cz plants and a few others and the dutch growers but if they are tc'ing sarracenia it must be seed as the hot varieties are not being sold anywhere unless they are building stock, but I think they just stick with mass produceding nameless varieties.

I think it will have to be down to private growers to work this out
By Tony C
Posts:  352
Joined:  Wed Mar 07, 2012 10:23 am
#145238
TC from some sort of non-seed tissue has been worked out, that is how we have cultivars like 'Judith Hindle', 'Tarnok', 'Daina's Delight', and the UNC Little Bugs series so readily available. The issue is that they were done by commercial labs who don't share their methods.
By cvlover
Posts:  447
Joined:  Thu Apr 23, 2009 4:59 pm
#145239
It's better to use Sar. seed for TC beginner. It's hard to sterilize explant not mulitply. Sar quickly multiplies without hormone. After a year you may have hundred or more if you want to multiply.

Image
From one seed, it can produce 5 jars like this

Image
Deflask and growing

Image
Deflask from one jar. There is not enough space to deflask 5 jars.
By GothicJackalPaws
Posts:  361
Joined:  Wed Apr 11, 2012 10:20 pm
#145242
cvlover wrote:It's better to use Sar. seed for TC beginner. It's hard to sterilize explant not mulitply. Sar quickly multiplies without hormone. After a year you may have hundred or more if you want to multiply.

Image
From one seed, it can produce 5 jars like this

Image
Deflask and growing

Image
Deflask from one jar. There is not enough space to deflask 5 jars.
I'm not entirely sure I understand the concept of tissue culture, and how it can produce all those plants from just one seed. Educate me? :?
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By Matt
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Joined:  Mon Apr 21, 2008 11:28 pm
#145258
Those are actually Darlingtonia in the photos :)
GothicJackalPaws wrote:I'm not entirely sure I understand the concept of tissue culture, and how it can produce all those plants from just one seed. Educate me?
It's exactly the same way that plants divide naturally when you grow them in dirt except you give them nutrients and hormones to make it happen more often.
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