rellenburg wrote:I can't imagine finding any better tissue. Some were large newly grown B52 leaves and some were fairly small from a fuzzytooth, but all died the same.
When did you harvest them? This time of year it's nearly impossible to find good tissue.
rellenburg wrote:Soak/shake in a baby food jar with 1 drop of original Dawn detergent for 2 min.
That could potentially damage the tissue quite a lot. I've noticed that certain kinds of soap, including Dawn, are much harsher on the tissue.
rellenburg wrote:Rinse in tea ball under running water for 2 min. 3 min hydrogen peroxide, 6 min 10%bleach, 2 dist. autoclaved water rinses. Trimmed off traps and small section of bottom of the leaves. Transfer to media???
Nothing there would have killed the tissue unless you got a lot of phenolic bleeding and didn't move or replate the tissue.