too much Agar,killing?

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HarrisAz

 
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too much Agar,killing?

by HarrisAz » Fri Nov 26, 2010 8:05 am

howdy!
a questions here.
if i put more Agar that it should be or instead of 7grams per litre,i put 20Gs,would anything bad happen?will the future plant in there grow just fine or they wont grow well?



Thanky
Harris ;)
Thanks,
Harris Azariel

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Frankco

 
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Re: too much Agar,killing?

by Frankco » Fri Nov 26, 2010 4:43 pm

Wow, 20gram. I think the gel would be hard as a rock with that much.

Agar produces a matrix which allows the liquid part of the media to flow through. A higher amount of gel the smaller the pathways in the gel. With 20 grams of agar very little lquid media, what little is left, will come in contact with the plants limiting the amount of nutrients and plant growth regulators the plants have access to.

It may not kill them right from the start but will certainly affect their growth.

Frank

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HarrisAz

 
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Re: too much Agar,killing?

by HarrisAz » Sat Nov 27, 2010 1:50 am

Owh....i tought more agar,more strenght on explant.


Thanks Frank!
Thanks,
Harris Azariel


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