FlytrapCare Carnivorous Plant Forums

Sponsored by FlytrapStore.com

Discuss all micropropagation related topics here.

Moderator: Matt

By goldslinger
Posts:  772
Joined:  Thu Jul 09, 2009 1:31 am
#69206
Buy 2 plastic tubs. One to store Your prepared jars in, and a much larger one that You can turn upside down and cut out a square opening in the side for culture transfers, etc. That one would be Your 'clean room.' Some People use a fish tank turned on it's side as well.

Carol at Kitchencultures.com has good start up kits complete with manual and I think a CD.
Last edited by goldslinger on Thu Sep 02, 2010 1:22 am, edited 1 time in total.
goldslinger liked this
By Hayden
#69211
Ive also just watched the video, he boils his media with the agar in the main mix. I get that you only add the agar in the pressure cooker stages, but do I have to boil the current mix?

Thankyou
By goldslinger
Posts:  772
Joined:  Thu Jul 09, 2009 1:31 am
#69359
All ingredients should be in the media before You boil it; sugar, Agar, MS, Vitamins, PGR'S (if You want) etc.

Agar type gelling agent isn't water soluable at all until boiled and requires cooking before dispensing to obtain uniformity of hardness or strength of Gelling properties from vessel to vessel. That is why People cook the prepared media. So technically You are cooking Your prepared media twice; once to dissolve the agar, and again in a pressure cooker, microwave, etc. for sterilization of the prepared media and the vessels holding it. This method gives You flexibility in how much media You want to dispense from vessel to vessel, knowing it will set up uniformly, irregardless of the amount. Agar gels upon cooling undisturbed.

You can get around the above problem of cooking twice by using a level 'smidgeon' measuring scoop per 25ml of media and dispense that little measure right into each of the vessels, swirl a bit and go into the autoclave which sterilizes and boils and dissolves the Agar all in one. You have to make sure You dispense the right amount of agar for the quantity of media in the vessel or You will end up too hard or soft on the media gel.

Carageenan type gelling agent, (I.E. GP-812) is quite popular as it is partially water soluable, dispersing uniformly so You can dispense into the vessels cold and only have to cook once (sterilization process) to activate it's gelling properties upon cooling.

With Carageenan type Gels, it's a good idea to occasionally stir the uncooked media while dispensing in the vessels, as the Carageenan can start to settle a bit making the first jars set up a little soft, and the last jars set up a little too hard.

No matter what Gelling agent You use, a little variance in hardness in media from vessel to vessel can be expected and is acceptable.

Hope this helps,

Gary
By Doomsday
Posts:  621
Joined:  Sun Mar 07, 2010 3:11 pm
#84385
I am NOT an expert or even a decent...spert in any way at all, but this is what you should get imo.

A ten-20 gallon fish tank for doing the tc in as your sterile zone.
Some lights to grow out the jars.
A pressure cooker.
Baby food jars.
Autoclaveable caps
By LSK
Posts:  33
Joined:  Wed May 12, 2010 4:02 pm
#86142
1 "smidgen" spoonful it's = to how much grams of agar?
By Noddy
Posts:  56
Joined:  Mon Jan 04, 2010 1:01 am
#94217
Can I ask a silly question here please

Is there a best light level, I know low lighting but the actual level that's best and duration in addition to that the Temperature that is also best for the plants...

Many thanks Noddy
User avatar
By Matt
Location: 
Posts:  20961
Joined:  Mon Apr 21, 2008 11:28 pm
#94229
Noddy wrote:Is there a best light level, I know low lighting but the actual level that's best and duration in addition to that the Temperature that is also best for the plants...
I have read a number of foot candles before, but I don't recall what it was. Even though they grow OK with less light, I use the same amount of light for my cultures as I do for my plants in vivo that I grow under artificial lights. I think that Venus fly traps do better with stronger light even in vitro.

As for temperature, anywhere between 72°F and 82°F is optimal. Any warmer than that and you can cook them. Cooler than that and they don't grow as quickly.
Matt, Matt liked this
By winterkid1
Location: 
Posts:  27
Joined:  Sat Jan 01, 2011 3:48 pm
#94401
hey matt .........................
do you do tissue culture at your nursery??????????
and if you do ...can you send it to malaysia?????
i am looking for rare vfts that i cant find in malaysia.................
looking for : dentate,fused tooth,wacky traps, bristle tooth and other intresting vfts....................
hope you can TC them and ship to malaysia..................^^
thx,
sam
User avatar
By Matt
Location: 
Posts:  20961
Joined:  Mon Apr 21, 2008 11:28 pm
#94423
winterkid1 wrote:hey matt .........................
do you do tissue culture at your nursery??????????
and if you do ...can you send it to malaysia?????
i am looking for rare vfts that i cant find in malaysia.................
looking for : dentate,fused tooth,wacky traps, bristle tooth and other intresting vfts....................
hope you can TC them and ship to malaysia..................^^
thx,
sam
Hi Sam,

Yes, I do tissue culture and I can ship to Malaysia or anywhere else in the world. You can purchase sterile cultures here:
http://www.flytrapcare.com/store/intern ... onaea.html

Remember that a Phytosanitary Certificate is required for EVERY shipment. Also, Malaysia requires you to obtain an import permit. If you don't know how to contact the Malaysian government, I can help you with that. You can find their contact information here:
http://www.flytrapcare.com/store/about-sterile-cultures
By jht-union
Location: 
Posts:  3205
Joined:  Tue Apr 13, 2010 11:43 pm
#94454
Dionae wrote:Will TCs ever be available to US customers?
I was wondering that too, the FTS international have more VFT's in TC for sale than plants available invivo for the U.S.A(so people not living in the U.S.A, consider yourself very lucky! ;) )
Can't wait when those are in stock for U.S.A people!
User avatar
By Matt
Location: 
Posts:  20961
Joined:  Mon Apr 21, 2008 11:28 pm
#94478
Dionae wrote:Will TCs ever be available to US customers?
Maybe, maybe not. I haven't decided yet.
jht-union wrote:I was wondering that too, the FTS international have more VFT's in TC for sale than plants available invivo for the U.S.A
That's because it takes a long time to plant out the tissue and grow it to a reasonable size for sale. Everything that's in stock in the FTS international store in vitro will eventually be for sale within the US in vivo (planted out).
By BradR
Location: 
Posts:  450
Joined:  Sun Nov 28, 2010 5:00 pm
#94489
Matt wrote:
Dionae wrote:Will TCs ever be available to US customers?
Maybe, maybe not. I haven't decided yet.
Don't do it Matt. You worked too hard getting all those clones into TC to hand them over to your competitors.

Just my two cents worth.
Brad
BradR liked this
  • 1
  • 4
  • 5
  • 6
  • 7
  • 8

I didn't do anything yet! Although my flytrap flow[…]

VFT from Disney Mickeys mini garden (Help!)

I live in NJ for reference. I'm in NJ too :D Yo[…]

Terrarium ac, too much?

Okay thanks I hope I haven’t been hurting[…]

You can tell if it has oreophila in it if the grow[…]

SASE received. Order is fulfilled. Return envelope[…]

0006 sanguinearocks101 requests P. Ionantha

I have received the seeds. Thank you.

Repotting and general advice

Hoping it won't do any harm to have her in a big […]

Nepenthes on a tree?

This is my favorite resource to estimate what temp[…]

Support the community - Shop at FlytrapStore.com!