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By vignesh_agumbe
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Posts:  29
Joined:  Sun Jun 30, 2024 11:31 am
#455602
I used this water cleaning chlorine tablet s for explant sterlization of bryophyllum sp. I kept it in 640ppm NaDCC or 400ppm free chlorine solution for 24h . it works well for this
But for nepenthes recommended is 300ppm for 12h -24h
Work well for drosera in 12h 300ppm NaDCC
nepenthes has endophyte which means organism living inside another organism

It can be eliminated by pre treatment of streptomycin and tetracycline
With pre treatment of systematic fungicide based on sp. Before taking explant in plant it self in the nodes
This i collected out of literature
Not tryed because nepenthes ventrata dont have endophytes in them
PPM also used to rinse it 10ml/l
And 2ml in media
By vignesh_agumbe
Location: 
Posts:  29
Joined:  Sun Jun 30, 2024 11:31 am
#455604
This experiment ended with bleaching the tissue but bryophyllum have the softest tissue
Considering that its fine
Attachments:
20240902_185429.jpg
20240902_185429.jpg (2.62 MiB) Viewed 266 times
20240902_190821.jpg
20240902_190821.jpg (3.15 MiB) Viewed 266 times
20240903_230506.jpg
20240903_230506.jpg (2.95 MiB) Viewed 266 times
By vignesh_agumbe
Location: 
Posts:  29
Joined:  Sun Jun 30, 2024 11:31 am
#456229
Nepenthes ventrata 300 ppm nadcc
Attachments:
Nepenthes ventrata
Nepenthes ventrata
20240928_161529.jpg (1.63 MiB) Viewed 86 times

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