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Discuss all micropropagation related topics here.

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By Greenthumbs Garden
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Posts:  636
Joined:  Mon Jul 27, 2009 10:15 pm
#383548
I'm about to order some Phytocaps, the vented ones for baby food jars. I'm a bit confused about the vents. I thought the cultures were completely sealed off from the outside world? but I read that they also need vents? So if i do use bigger jars and seal the lids additionally with parafilm, do these need vents adding and micron tape?
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By Greenthumbs Garden
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Posts:  636
Joined:  Mon Jul 27, 2009 10:15 pm
#383629
@Matt, Ah ok, so even if I buy some jam jars with good lids like I posted once before, then use PVC or parafilm to seal them even further then I should be golden? What's the benefit of vented lids? and if I'm going to buy a few phytocaps, then might I just as well get the vented version? or is it better to stick with no vent. sorry a lot of questions, lol.
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By Matt
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Joined:  Mon Apr 21, 2008 11:28 pm
#384727
Greenthumbs Garden wrote:@Matt, Ah ok, so even if I buy some jam jars with good lids like I posted once before, then use PVC or parafilm to seal them even further then I should be golden?
Yep, for sure.
Greenthumbs Garden wrote:What's the benefit of vented lids?
From what I recall, the ventilations helps the plants get the air exchange they need to grow better. But I've never had any issue with sealing the jars up tight, not venting them at all, and even putting them inside plastic baggies.
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By Greenthumbs Garden
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Posts:  636
Joined:  Mon Jul 27, 2009 10:15 pm
#384782
@Matt, Brilliant! I made and paid for my order last week and they say they should be sending it this coming week so I'm super excited. Here is what I ordered;
Catalog No. 30 (Plant Preservative Mixture (PPM) 30ml) PPM30
Murashige & Skoog Vitamin Powder (1000x) “Product ID M533”
https://phytotechlab.com/murashige-skoo ... 000x.html

C070 CLOSURE, PHYTOCAP x25

Forceps, the 8inch “dressing”

Sealing Film, Parafilm, 2in x 125ft

Mediums:
Orchid Seed Sowing Medium “Product ID P723” 1L
https://phytotechlab.com/orchid-seed-so ... p723.html

Orchid Maintenance Medium with Charcoal, without Agar “Product ID P668” 10L
https://phytotechlab.com/orchid-mainten ... agar.html

Carnivorous Plant (Nepenthes) Basal Salt Mixture “Product ID C1935” 10L
https://phytotechlab.com/carnivorous-pl ... ture.html

M5531-10L M & S MODIFIED BASAL MEDIUM (Vitamins + Mesa)


So I just want to order an electronic pH meter from Amazon and maybe a scalpel. I have a small pressure cooker here but it has no psi gauge or temp meter... but don't want to fork out for a big pot just yet. A also think Ill build a glove box like this guy is using in the video sowing orchid seed. Been watching his series which is giving me a plan. I think I'll make a similar glove box but chop the bottom out a little and silicone on some clear perspex at a 45o angle to give better viewing.
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