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By Cross
Posts:  1445
Joined:  Fri Oct 26, 2018 11:25 pm
#361440
So, originally I couldn't find a bunch of baby food jars. I bought a bunch of the clear square containers from phytolabs. I think my environment is just not sterile enough because inevitably I always end up with mold. Well I spent the money and bought the baby food jars (I forgot how expensive they were in store lol). I had bought the lids Matt mentioned in his article, but if I don't have enough can I cover the jars with something else? I mean I could put the original lids on after it goes through the pressure cooker, but what else can I cover them with during the pressure cooker stage? Shipping is just crazy expensive from phyto. Thanks in advance.

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By optimus prime
Posts:  850
Joined:  Mon Jun 22, 2020 12:26 am
#361441
Cross wrote: Sun Aug 02, 2020 12:59 pm So, originally I couldn't find a bunch of baby food jars. I bought a bunch of the clear square containers from phytolabs. I think my environment is just not sterile enough because inevitably I always end up with mold. Well I spent the money and bought the baby food jars (I forgot how expensive they were in store lol). I had bought the lids Matt mentioned in his article, but if I don't have enough can I cover the jars with something else? I mean I could put the original lids on after it goes through the pressure cooker, but what else can I cover them with during the pressure cooker stage? Shipping is just crazy expensive from phyto. Thanks in advance.

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A paper towel attached to the top will work. It will hold moisture but also allow air to flow though. If you jawing these for seeds I would recommend using these little dip cups.
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By Copper2
#361479
Optimus, I am afraid that you do not understand.

Cross, I take it that you are experiencing contamination the majority of the time after the two week stage, am I correct? The clear square containers from phytotech are very good along with their other autoclavable flask and lid combo packs. What is happening though is even though you screw or push the lid down onto the flask there is still airflow going through that tiny gap between the lid and flask and that’s where the bacteria are getting in. So after plating flask you need to seal the lid with some clear tape stuff phytotech has or electric tape and that will give you an airtight seal. It’s always better to be airtight since airflow will dry out the media too
By Copper2
#361482
Sealed flask using electric tape
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