My TC adventure

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Re: My TC adventure

by tokol0sh » Sat Aug 31, 2019 4:12 am

Thanks for the reply.
My media is 1/3 MS with 3% sucrose.
My sterilization protocol was:
Dip in IPA, 10min soak in 10% bleach and rinsed 3 times in sterile DI water (I didn't note how long each rinse was). I figured I might have over sterilized the seeds and tried an 8min soak in the bleach solution with the same results.

There's no signs of contamination, Ill just leave them alone for a month or so longer and see of anything happens.


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