Excess moisture

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Excess moisture

by Lorib_nc » Fri Dec 07, 2018 1:47 am

Hi all. I'm new to the forum and tissue culture. I've recently gotten my first batch of nepenthes seeds into the culture vessels. 48 hours later there is a pool of fluid on top of the agar. The seeds appear to be floating on the liquid. I can't find anything about excess moisture in any of the texts I've been learning from. Is this something I should be concerned about? Is it too late to do anything about it?

Lori B in NC

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Re: Excess moisture

by Eteson » Sat Dec 08, 2018 12:55 pm

Hi Lori, do not worry too much. This happens sometimes but for me has not been a problem.
How much gelling agent did you used?

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Re: Excess moisture

by mantis » Mon Dec 10, 2018 11:49 am

I let my medium solidify while the jars are slightly tilted.

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