FlytrapCare Carnivorous Plant Forums

I have been attempting to Tissue Culture venus flytraps for a while now and I have had little success. The explants are usually young and healthy and remain green after treatment with disinfectants, however, they only elongate and do not form calli. I have tried the flytrapcare's tissue culture media recommendations (1 mg/ml bap half ms, full vitamin) and Kinetin media recommendations (2.3 uM kinetin) as shown by Gi-Won Jang, et-al. These recommendations resulted in elongation, but failure to produce calli even after 2 plus months. All samples were kept on a 16 hour photoperiod. My questions are as follows:

Should I increase the Kinetin and BAP concentrations to promote calli induction?
Has anyone tried using 2IP for plant tissue culture of flytraps?
How long do you wait before seeing callus induction?

I volunteer in a plant biology lab at BYU-I. We are attempting to create a polyploid venus flytrap that will hopefully produce huge traps.

Cam

a-priori wrote:Should I increase the Kinetin and BAP concentrations to promote calli induction?

I wouldn't recommend it. Callus should form on Dionaea tissue even without any hormones. With hormones, it can take a while for the plants to begin growing normally again after they take hold and form a successful culture.

a-priori wrote:Has anyone tried using 2IP for plant tissue culture of flytraps?

Not me. I've never tried it.

a-priori wrote:How long do you wait before seeing callus induction?

Sometimes it happens in 6 to 8 weeks. Sometimes it takes 4 to 6 months. Sometimes it never happens even if you have good tissue and are successful in getting it clean without killing it.

We use C206 media from phytotech to get them going. It has 2IP in it, works great.

nicszabo wrote:We use C206 media from phytotech to get them going. It has 2IP in it, works great.

awesome. how long does it take for calli to appear?

Hey there Cam!
What protocal do you use to produce the 16 hour photo period?

1cashew wrote:Hey there Cam!
What protocal do you use to produce the 16 hour photo period?

I put the, in the grow room at our university.
I don’t know that there is a protocol for this.
I keep them about a foot from the lights, but they don’t need super intense light apparently.