Let the games begin!

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Doomsday

 
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Re: Let the games begin!

by Doomsday » Tue Feb 07, 2017 4:57 am

tannerm wrote:Look good!


Sent from my iPhone using Tapatalk


Thanks!
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Matt
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Re: Let the games begin!

by Matt » Tue Feb 07, 2017 4:35 pm

Yes, very nice!

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Doomsday

 
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Re: Let the games begin!

by Doomsday » Wed Feb 08, 2017 3:31 am

Matt wrote:Yes, very nice!

Thank you, Matt!


I've got a new experiment going:

Drosera sp. "Floating" in hormone-free media...
Image
Image
Image

...and it's root cuttings, on shooting media.
Image
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Matt
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Re: Let the games begin!

by Matt » Wed Feb 08, 2017 4:46 am

Awesome! Keep us up to date.

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Re: Let the games begin!

by David F » Wed Feb 08, 2017 6:28 am

I like the look of your culture vessels.
Looking for any drosera capensis via seed, pm me!
I'm interested in any sundews.

Growlist/trading profile:
http://www.flytrapcare.com/phpBB3/david-f-s-growlist-t26539.html

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Re: Let the games begin!

by Doomsday » Wed Feb 08, 2017 7:29 am

Matt wrote:Awesome! Keep us up to date.

I will! Hopefully the roots strike quickly!

David F wrote:I like the look of your culture vessels.

Thanks. These cube ones are MK-5 vessels from Caisson Labs, I like them for pictures and general viewing. They are much easier to look through than rounded glass.
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Doomsday

 
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Re: Let the games begin!

by Doomsday » Fri Feb 10, 2017 8:32 pm

This Dionaea callus is starting to look more and more like an animal, every day.
Image

Image

Image
Last edited by Doomsday on Fri Feb 10, 2017 8:56 pm, edited 1 time in total.
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Re: Let the games begin!

by bijillon » Fri Feb 10, 2017 8:36 pm

What is it? I know it's a callus


Happy Growing,

Dillon

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Re: Let the games begin!

by Doomsday » Fri Feb 10, 2017 8:59 pm

bijillon wrote:What is it? I know it's a callus


Happy Growing,

Dillon

Well, it was a mass of undifferentiated cells (callus) before, on hormone containing media, but since being switched to hormone-free media, the cells are being differentiated (turning into root and maybe shoot tissue) which is causing the root hair growth, and white/green/red blobs.

I'm hoping to be able to get some plants out of it within the next few months.
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Re: Let the games begin!

by bijillon » Sat Feb 11, 2017 1:55 am

So it a ball of root and leaf tissue together and they'll separate?


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Dillon

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Re: Let the games begin!

by Doomsday » Sat Feb 11, 2017 7:24 am

bijillon wrote:So it a ball of root and leaf tissue together and they'll separate?


Happy Growing,

Dillon

Currently, yup! I'm hoping they start to form individual plants that I can break apart, but I might have to chop it up depending on how it continues growing. I might even have to put it on more hormone to get little plant shoots to form, but it looks like that might be unnecessary, with how well it's been differentiating the newly growing tissue.

It's wild looking, isn't it?!
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Re: Let the games begin!

by Matt » Mon Feb 13, 2017 6:39 pm

Doomsday wrote:This Dionaea callus is starting to look more and more like an animal, every day.

That's really bizarre looking!

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Re: Let the games begin!

by coffeenflowers » Tue Feb 14, 2017 11:57 pm

Uh. So admittedly I'm not familiar with plant tissue culture but I do mammalian and insect cell culture for my job and my first thought is that the fuzzy brown stuff is contamination (aka mold).

Crystal
grow list: viewtopic.php?t=32983

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Re: Let the games begin!

by Doomsday » Wed Feb 15, 2017 5:48 pm

Matt wrote:
Doomsday wrote:This Dionaea callus is starting to look more and more like an animal, every day.

That's really bizarre looking!

Isn't it?! The thing just keeps transforming. I'm curious to see what comes out of it

coffeenflowers wrote:Uh. So admittedly I'm not familiar with plant tissue culture but I do mammalian and insect cell culture for my job and my first thought is that the fuzzy brown stuff is contamination (aka mold).

Crystal
grow list: viewtopic.php?t=32983

I would've thought so, expect it's not spreading to the media, and the hair's formed on the part of the callus that was originally going to be the seedlings root.
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Doomsday

 
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Re: Let the games begin!

by Doomsday » Thu Feb 16, 2017 4:28 am

Dionaea seedlings:
Image

Here are some little Drosophyllum lusitanicum offshoots that were growing out of my main plants. I replated them onto shooting media:
Image
Image
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