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Discuss all micropropagation related topics here.

Moderator: Matt

By nitronugget
Hi all, I have noticed that I can get this MURASHIGE & SKOOG MODIFIED BASAL SALT MIXTURE W/OUT N
which is without nitrogen which I believe Neps dont like. Am I right in thinking this would be an excellent medium to use? and would it be ok for all CP's?
By cvlover
N is the main nutrient for plant and without it plant cannot grow up. Neps may required less N than other plants (?) but it should be in the media. We grow many Neps with 1/3 MS without any problem.

nep1.jpg (420.5 KiB) Viewed 2740 times

A healthy Nep in 1/3 MS.
By nitronugget
Ok thanks, are you using 1/3 MS and full vitamins? As i am using MS with vitamins at 1/3 strength and am just wondering if everyone runs plain MS but with the full vitamins and if so how is this acheived.

Thanks in advance :)
By cvlover
Try full amount of vitamins and micro-nutrients if you can. Use 1/3 MS with vitamin and 2/3 vitamins (or 1/3 MS - no vitamin with full vitamins); 1/3 MS with 2/3 micro-nutrients. Some articles said it's better so I try but I don't see much different; anyway it gives you more confident.
By DontTazeMe
I want to do like a 2 month long tissue culture experiment on venus fly traps in tissue culture, each in different jars, with diff MS, diff vitamins, diff sucrose, and combo of hormones and try and debunk some of these recipes have, and verify others.
By nitronugget
Cool thx cvlover. Is there any particular vitamins product i should buy? Ill have to go have a look what there is available. What exactly am i looking for?
By cvlover
Today, most people use Gamborg vitamins for plant tissue culture. Let take a look at the formula:
Thiamine (Vitamin B1) --------- 10.0 mg/liter
Pyridoxine (Vitamin B6)-------- 1.0 mg/liter
Nicotinic acid (Niacin) --------- 1.0 mg/liter
Myo-inositol --------------------- 100 mg/liter

Vitamin B1 is the most important for plant cells. You can buy Gamborg vitamins at Phytotech, Sigmaaldrich, and Caison. You can make yourself 100ml 100X vitamin solution:
• Dissolve 1 Nature Made super B complex (B1 100mg, B6 2mg, niacin 25 mg) in 100 ml distilled water. Each 1 ml has 1 mg vitamin B1, Use 10 ml for 1liter media you have 10mg/liter B1 + B6 and Niacin which is almost the same with Gamborg.
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