FlytrapCare Carnivorous Plant Forums

Hi,
please could you share your favorite sterilization protocol for Dionaea flower stalk ? Anyone have success with NaDCC ?

Milos

Thanks Matt,
i will try it. I have tried only 10% bleach alone before for upto 20minutes, but i have found everything was contaminated. I have tried then 20% bleach for 10 minutes and everything was clean and green for one month. Then all explants start to die without any signs of regeneration ( 1/3MS + 1mg/l kinetin ).

I mite be a good idea , what Matt briefed over, take your plant and cover it when new growth is happening before you attempt to take any ex-plants of any kind min or 2 months before hand, only bottom water and keep covered , to lessen any water, dust and alike settling on the plant making it hard to clean , cleaner the new growth is easier it is to sterilize and when the flower stalk is starting it will be much better to work with
J

With Matt's protocol you get either 100% or going to 80% sterilization, then the flower stalk some tend to die and some to grow, with my experience with 7 pieces about 2cm I got 2 or maybe 3 to regenerate like 100 plants in total, they took kind of almost 3 months to do so, and slowly the others died.