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Discuss all micropropagation related topics here.

Moderator: Matt

By jht-union
Posts:  3205
Joined:  Tue Apr 13, 2010 11:43 pm
#116239
Dubstep13 wrote:where is the best place i can get a TC starter kit for germinating VFT seeds and growing them for a bit to the size of a half dollar?

Here's the solution :) :
http://hometissueculture.org/catalogkits.htm
By jht-union
Posts:  3205
Joined:  Tue Apr 13, 2010 11:43 pm
#118594
My first explant success!!!!!!!!!!!!!!! :D. For being my second attemp, i can say i'm proud! I will try to post pics whenever i can! :)

I see a mass of tissue(callus), in the media!
jht-union liked this
By jht-union
Posts:  3205
Joined:  Tue Apr 13, 2010 11:43 pm
#120041
gpblakely wrote:Do you have nescofilm or parafilm around the
containers?
No, but i wrap the container in wrapping paper to prevent particles from scrawling the edges and reaching the sterile zone. (sorry for the late replay :( )




And here's another success! :) :

Image

Image

Image

Image

I think 1-2 more explants are forming callus in other containers! :)

So far is going very well! I still have to fill some 60 something vessels thought, but i will do that sometime soon. :)

Thanks for looking at my progress! :)
jht-union liked this
By jht-union
Posts:  3205
Joined:  Tue Apr 13, 2010 11:43 pm
#121929
Thanks daniel,

Yup, i have a tip for you, be as tedious as possible, and remember to clean the tools when dealing with every explant, you want to remember to clean every single tool that are touching one explant to then go to the next explant.

Good luck!

:)

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