- Mon Jul 31, 2017 5:09 pm
Yeah, you'll want to cut the MS by 1/3 (or 1/2), but then I would expect your media will be deficient on PPM and vitamins. Still use full sugar (you probably want 2 heaping tbsp) and full agar. All you could do then is get your own PPM and vitamins and add 2/3s of each to your solution to even it out. Without doing that, you'll have to keep it very sterile because the PPM won't be strong enough to do much, if anything. You should still see growth without full vitamins, but the growth might either not be optimal, or you might just need to re-plate more frequently, technically leaving some of the MS nutrients in the media unused...which isn't a big deal unless you were running a large-scale commercial operation.
S. leuco "H.C. White"
D. muscipula: "Microdent", "Dentate"
C. follicularis: Hummer's Giant