- Sat Jul 29, 2017 6:16 pm
#299513
Hello all, I ordered a tissue culture kit from Monster Gardens and they have now started to mix the MS into a solution so that what you recieve is the solution of MS and the Agar, there is no powder to mix in it besides the Agar. So that all you have to do is mix in the solution of MS, 2 TBL spoons of sugar, and 1 packet of Agar into 1 liter of water and you're done. I know that the premixed solution has MS and PPM in it but I'm not sure if there anything else in it. So I have read that most CPs like 1/3rd to 1/2 of the normal MS nutrients for thier medium. So my question is do I simply mix in only 1/3 or 1/2 of the premixed MS solution and do the rest as I'm supposed to? I assume the amount of Ager is the same but do I cut down the sugar too? Is there anything critical in the premixed solution so that if I don't add it all in will ruin the medium? Also, will diluting the PPM affect the TC's chances? I hope this question makes sense. Thanks in advance for any help.
Last edited by whatsgrowingon on Sat Jul 29, 2017 6:30 pm, edited 1 time in total.