- Thu Dec 03, 2020 5:13 pm
VFT's never work using explant; can only realistically use in-vitro divs or seeds. Seeds are not a bad way to go, order some from Matt/Leah at FTS. Then, a 5 min soak in 5% bleach, move it to methanol/ethanol (Pure), then into an SDS solution ( I have used dishsoap). Then, rinse 4-5 times using autoclaved/microwaved sterile water. Plus, seeds grow pretty fast.
For me, the best and only explants I have taken are Heli pitchers. Rip them off, sterilize, and stab into media.
Did you use PPM? If so, I'd be down to trade plants with you for some supplies like agar, MS, and ppm.