- Sun Nov 23, 2014 8:24 pm
#217781
Hi there
I'm planning on culturing some CPs for the first time and was hoping someone could help me with a few questions. My starting point is some Darlingtonia seeds. I have experience working with cell cultures, sterilizing equipment, making media and working in a laminar flow hood, but this would be my first time doing micropropagation.
My first question is what makes an ideal culture flask and lid? I was going to use small jam jars or baby food jars with their original lids and use tape to seal them up. If I do that, should I have an air vent stuffed with non-absorbent cotton wool to allow for gas exchange?
Also, once the darlingtonia seeds are in the media, how often should you transfer to fresh media and what source and intensity of light should I use? Is there an optimum temperature for Darlingtonia as well?
Excited about giving this a go and would be very grateful for any advice you can give.
Cheers,
Mark
I'm planning on culturing some CPs for the first time and was hoping someone could help me with a few questions. My starting point is some Darlingtonia seeds. I have experience working with cell cultures, sterilizing equipment, making media and working in a laminar flow hood, but this would be my first time doing micropropagation.
My first question is what makes an ideal culture flask and lid? I was going to use small jam jars or baby food jars with their original lids and use tape to seal them up. If I do that, should I have an air vent stuffed with non-absorbent cotton wool to allow for gas exchange?
Also, once the darlingtonia seeds are in the media, how often should you transfer to fresh media and what source and intensity of light should I use? Is there an optimum temperature for Darlingtonia as well?
Excited about giving this a go and would be very grateful for any advice you can give.
Cheers,
Mark