Page 1 of 1

Cephalotus on NAA

Posted: Fri Jan 29, 2010 5:10 pm
by nepaholic
Hi
here some pics i took today.
the left culture contains NAA and the right not. Both have 20%MS
Image

Image

Image

Re: Cephalotus on NAA

Posted: Fri Jan 29, 2010 6:38 pm
by Matt
Looks like the NAA is working for rooting the Cephs.

Did you have problems deflasking them without roots?

Re: Cephalotus on NAA

Posted: Fri Jan 29, 2010 6:54 pm
by nepaholic
Yes Matt it seems that they grow bigger and get more roots. I don't have any Problems to plant them out and it Survive arround 99% ;)
I just want bigger plants

Re: Cephalotus on NAA

Posted: Fri Jan 29, 2010 7:11 pm
by Matt
nepaholic wrote:Yes Matt it seems that they grow bigger and get more roots. I don't have any Problems to plant them out and it Survive arround 99% ;)
I just want bigger plants
Ah, ok. I thought you had a good survival rate when planting them out. But it's always good to have larger plants when you deflask!

Re: Cephalotus on NAA

Posted: Fri Jan 29, 2010 7:41 pm
by Bud
Very Nice Jens, and great Photos!- always enjoy them.

What did you use for your initial media for the explants? I assume you used NAA after replating? Also were the explants difficult to disinfect?

Thanks Much!

Bud

Re: Cephalotus on NAA

Posted: Fri Jan 29, 2010 7:53 pm
by goldslinger
More roots, more absorbtion, better growth.

I wonder if this is the correlation?

NAA is just a rooting hormone, no nutritional value or stimulus on anything else?

Re: Cephalotus on NAA

Posted: Fri Jan 29, 2010 8:19 pm
by nepaholic
Bud i didnt introduced the cephalotus in TC i got à Culture several months ago from a friend.

Re: Cephalotus on NAA

Posted: Fri Jan 29, 2010 9:21 pm
by ahicks51
goldslinger wrote:More roots, more absorbtion, better growth.

I wonder if this is the correlation?

NAA is just a rooting hormone, no nutritional value or stimulus on anything else?
NAA is an auxin; like allied compounds (IBA, IAA), it inspires root growth. Kind of like with drugs that work on the mammalian autonomic nervous system (the old "alpha, beta, constriction, dilation thing), there are no absolutely pure forms of auxins and cytokinins- although some come very close. There's usually some overlap in effects.

But! For getting plants to throw better roots, NAA is tough to beat. Rule of thumb with all hormones: start at 1 ppm (1 mg/L), and work outwards to one order of magnitude (0.1 mg to 10.0 mg/L) until the desired effect is achieved. With some exceptions, this range works for most plant hormones in most crops.

Re: Cephalotus on NAA

Posted: Fri Jan 29, 2010 9:31 pm
by hackerberry
Very nice. I see the same type of root when I try to do a cutting and dip the end in Rooting hormones [Rootone]. Cute tiny roots!

hb