- Sat Nov 21, 2015 4:33 am
#250936
So a week or so ago i bought a ceph culture... had about 16 plants in it which i plated out.... couple days later there was a bit of a heat wave, and they took a massive amount of damage... thankfully most of the larger plants still had lime green growth in them..
anyway, i have bought a second culture too... poor impulse control, had many more plants in it... and they were still clumped up so i had to cut them apart... probably got 30+ plants from them
i have also bought a wine cooler with a glass door, it seems on the lowest setting to stay around 18-22c inside... which seems pretty good to me.. here's a pic
doing some research, i came across this thread by bigbella http://www.flytrapcare.com/phpBB3/eden- ... cephalotus ... had some incredible callus growth in it, so i asked for some advice on how to achieve the same... was suggested upwards of 2ml/l bap for multiplication, so i have now approximately half of the new culture plated into 3ml/l bap media, and i want to log the different growth traits for the recovering cultures, and both the new cultures with and without BAP, so i will post pics here.
i currently have everything in the cool chamber, where they will stay for the next 6 weeks in darkness to reduce bleeding..
i have picked out 1 sample from each category to track... tried to get ones of reasonably similar size
recovery culture (this one is one of the ones that fared the hot weather the best... alot were more damaged)
multiplication culture (it's fully green... post processing just makes it look brown)
regular media
anyway, i have bought a second culture too... poor impulse control, had many more plants in it... and they were still clumped up so i had to cut them apart... probably got 30+ plants from them
i have also bought a wine cooler with a glass door, it seems on the lowest setting to stay around 18-22c inside... which seems pretty good to me.. here's a pic
doing some research, i came across this thread by bigbella http://www.flytrapcare.com/phpBB3/eden- ... cephalotus ... had some incredible callus growth in it, so i asked for some advice on how to achieve the same... was suggested upwards of 2ml/l bap for multiplication, so i have now approximately half of the new culture plated into 3ml/l bap media, and i want to log the different growth traits for the recovering cultures, and both the new cultures with and without BAP, so i will post pics here.
i currently have everything in the cool chamber, where they will stay for the next 6 weeks in darkness to reduce bleeding..
i have picked out 1 sample from each category to track... tried to get ones of reasonably similar size
recovery culture (this one is one of the ones that fared the hot weather the best... alot were more damaged)
multiplication culture (it's fully green... post processing just makes it look brown)
regular media
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