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By Savior7
Posts:  29
Joined:  Mon Jul 27, 2015 9:10 am
#282227
Hello Everybody,

I just wanted give you some impression of some of my cultures which i own :-)

Best regards
Lukas

Nepenthes:
my main culture of Nepenthes Glabrata
my main culture of Nepenthes Glabrata
20161219_130619_1482410444280_resized.jpg (172.59 KiB) Viewed 8965 times
some Nepenthes Ampullaria 'red' currently on multiplication media
some Nepenthes Ampullaria 'red' currently on multiplication media
20161210_114345_1482410445956_resized.jpg (224.58 KiB) Viewed 8965 times
germinating Nepenthes Veitchii 'Giant'
germinating Nepenthes Veitchii 'Giant'
20161204_163222_1480929383159_resized.jpg (146.02 KiB) Viewed 8965 times
Some Nepenthes Viellardii
Some Nepenthes Viellardii
20161204_163156_1482410447962_resized.jpg (156.83 KiB) Viewed 8965 times
Some callus of Nepenthes Glabrata
Some callus of Nepenthes Glabrata
20161204_163116_1480929384237_resized.jpg (136.5 KiB) Viewed 8965 times
Heliamphora:
A Culture of Heliamphora Minor
A Culture of Heliamphora Minor
20161204_163139_1480929385277_resized.jpg (159.36 KiB) Viewed 8965 times
Dionaea:
a clump of Dionaea 'Schuppenstiel 1'
a clump of Dionaea 'Schuppenstiel 1'
20161219_130802_1482410443284_resized.jpg (159.13 KiB) Viewed 8965 times
Dionaea 'B52' from the top
Dionaea 'B52' from the top
20161219_130702_1482410442149_resized.jpg (164.74 KiB) Viewed 8965 times
Dionaea 'B52' sideview
Dionaea 'B52' sideview
20161219_130719_1482410440609_resized.jpg (215.81 KiB) Viewed 8965 times
a culture of some Dionaea 'cupped trap'
a culture of some Dionaea 'cupped trap'
20161210_114509_1482410445155_resized.jpg (254.07 KiB) Viewed 8965 times
Drosera:
a selected clone of Drosera Capensis 'wide leaf'
a selected clone of Drosera Capensis 'wide leaf'
20161210_114240_1482410446958_resized.jpg (310.86 KiB) Viewed 8965 times
a clone of Drosera Tokaiensis
a clone of Drosera Tokaiensis
20161204_163244_1480929386460_resized.jpg (264.57 KiB) Viewed 8965 times
Cephalotus Follicularis:
a culture of Cephalotus Follicularis 'Typical'
a culture of Cephalotus Follicularis 'Typical'
Cephalotus Follicularis.JPG (99.75 KiB) Viewed 8965 times
Savior7, Savior7, Savior7 and 1 others liked this
User avatar
By Shadowtski
Location: 
Posts:  4721
Joined:  Tue Mar 22, 2016 8:19 am
#282229
Good job there! Have you ever tried to culture any species in the Drosera indica complex? I bought D. hartmeyerorum from two different sources and both times the plant dissolved into grey goo in about two weeks time. I washed and rinsed it repeatedly for 30 - 45 minutes. I planted it in sterilized media and pfffttt it was gone.

I used the same procedure on deflasking Drosera slackii and it survives and is growing for me. The only difference I see is slackii is a Perennial and hartmeyerorum is an Annual.

Any thoughts?

Good growing,
Mike
By Savior7
Posts:  29
Joined:  Mon Jul 27, 2015 9:10 am
#282246
Hey Mike :-)

Thank you for your kind words!

Unfortunately i haven't made any expirience with drosera of the indica complex.

What media do you use for them ?

What about shipping ? Maybe the temperatures were too low for them so they died. Happened also to me once.

Best regards
Lukas
User avatar
By Shadowtski
Location: 
Posts:  4721
Joined:  Tue Mar 22, 2016 8:19 am
#282264
Savior7 wrote:Hey Mike :-)

Thank you for your kind words!

Unfortunately i haven't made any expirience with drosera of the indica complex.

What media do you use for them ?

What about shipping ? Maybe the temperatures were too low for them so they died. Happened also to me once.

Best regards
Lukas
Medium was 1 part peatmoss to 3 parts silica sand. I sterilized it in a pressure cooker 15psi for 45 minutes, then let it cool for several hours. Plant was only watered with distilled water. I ordered them in May and June, from the Czech Republic, so freezing shouldn't have been an issue.

They looked OK at first. Then they kind of went translucent. Then they changed from Green to Green-Grey, then to Grey. Then they melted into a puddle of Grey goo. It was frustrating because I didn't have a clue what I was doing wrong. I still don't know where my error was. After two catastrophic failures, I said the heck with it and concentrated on raising hartmeyerorum from seed.

Good growing,
Mike
By Savior7
Posts:  29
Joined:  Mon Jul 27, 2015 9:10 am
#282690
Shadowtski wrote:
Savior7 wrote:Hey Mike :-)

Thank you for your kind words!

Unfortunately i haven't made any expirience with drosera of the indica complex.

What media do you use for them ?

What about shipping ? Maybe the temperatures were too low for them so they died. Happened also to me once.

Best regards
Lukas
Medium was 1 part peatmoss to 3 parts silica sand. I sterilized it in a pressure cooker 15psi for 45 minutes, then let it cool for several hours. Plant was only watered with distilled water. I ordered them in May and June, from the Czech Republic, so freezing shouldn't have been an issue.

They looked OK at first. Then they kind of went translucent. Then they changed from Green to Green-Grey, then to Grey. Then they melted into a puddle of Grey goo. It was frustrating because I didn't have a clue what I was doing wrong. I still don't know where my error was. After two catastrophic failures, I said the heck with it and concentrated on raising hartmeyerorum from seed.

Good growing,
Mike
Hey Mike, sorry for my late reply just been busy the last few days.

So i didn't understand you correct. I thought you were replating these onto fresh Media for tissue culturing. Unfortunately i never expirienced something like this. I guess you lowered the Humidity slowly and started from about nearly 100% ?

What about the light ? Maybe it was a little bit too strong for them. I usually have very low light at the beginning of the acclimation process.

But some cultures aren't that easy to acclimate and have not the survival rate of a batch of D. Capensis. :-/
Doomsday wrote:Very nice! Were those nep cultures from seed?
Thank you Doomsday :-) Some of them yes. I raised the ampullaria 'red' and Veitchii 'Giant' from Seeds. I also own a N. Inermis which i also raised from seeds. All of the other Nepenthes shown i got from trades or ordered online.

Cheers
Lukas
By Savior7
Posts:  29
Joined:  Mon Jul 27, 2015 9:10 am
#283111
Hey Guys, wish you all a happy new Year!
Doomsday wrote:You're welcome! And, sweet! Good work. I like the Heli as well. What vessels are you using? They seem to work really nicely for pictures.
Thank you Doomsday :-)
These are PP Vessels i got them from here:
http://www.eimerundbecherprofi.de/index.php/cPath/2_5
But i guess they only deliver within germany/ austria.


I thought why not use the chance of these few days off and give you some updated Pictures.

So here we go:
Here's a Heliamphora Minor which i got a while back ago of a friend. Lovely to grow these in TC.
Here's a Heliamphora Minor which i got a while back ago of a friend. Lovely to grow these in TC.
H. Minor 02-01-16.JPG (40.22 KiB) Viewed 8756 times
Here's a shot of one of the D. Spatulata var. Lovellae Cultures. These grow like craazy. Way faster than i can replate them to seperate them.
Here's a shot of one of the D. Spatulata var. Lovellae Cultures. These grow like craazy. Way faster than i can replate them to seperate them.
D. Spatulata 02-01-17.JPG (95.37 KiB) Viewed 8756 times
This one's a Callus i subcultured from my main Glabrata Culture with a looot of shoots. Growing very nicely for me :-)
This one's a Callus i subcultured from my main Glabrata Culture with a looot of shoots. Growing very nicely for me :-)
N. Glabrata Callus 02-01-17.JPG (24.31 KiB) Viewed 8756 times
This one's my main N. Glabrata culture with five shoots. I got one more of these guys.
This one's my main N. Glabrata culture with five shoots. I got one more of these guys.
N. Glabrata 02-01-17.JPG (42.15 KiB) Viewed 8756 times
Here's a shot of one of my B52 Cultures. I really enjoy this cultivar! rapid growth on him. Can't wait to acclimatise these one day :-)
Here's a shot of one of my B52 Cultures. I really enjoy this cultivar! rapid growth on him. Can't wait to acclimatise these one day :-)
D.M. B52 02-01-17.JPG (800.75 KiB) Viewed 8756 times
Here's how my selected clone of capensis 'wide leaf' looks now! Take a look at that amazing root system even tough this is plain MS without any Hormones.
Here's how my selected clone of capensis 'wide leaf' looks now! Take a look at that amazing root system even tough this is plain MS without any Hormones.
D. Capensis wide leaf 02-01-17.JPG (1013.11 KiB) Viewed 8756 times
Hope you enjoyed them :-)

Cheers
Lukas
By massyudha
Posts:  1
Joined:  Wed Feb 22, 2017 3:33 am
#286824
Hello there savior7,
what a beautiful cultures you have there, what medium do you use for the plants?
By Savior7
Posts:  29
Joined:  Mon Jul 27, 2015 9:10 am
#296210
Hey guys,

I haven't made an update for a while so here are some random pics which i took over time.
massyudha wrote:Hello there savior7,
what a beautiful cultures you have there, what medium do you use for the plants?
I use for all of my plants some MS based medias :-).
some germinating Nepenthes seeds :-)
some germinating Nepenthes seeds :-)
IMG_20170318_205229_262_1498122084123.jpg (71.11 KiB) Viewed 8242 times
A dionaea explant on multiplication media after a two months :-)
A dionaea explant on multiplication media after a two months :-)
IMG_20170508_213647_085_1498122084375.jpg (89.93 KiB) Viewed 8242 times
some d. tomentosa var. tomentosa i grew from seeds. really fast grower in TC.
some d. tomentosa var. tomentosa i grew from seeds. really fast grower in TC.
IMG_20170508_214108_771_1498122084612.jpg (90.16 KiB) Viewed 8242 times
A heliamphora hybrid i grew from seeds and now placed on multiplication media :-)
A heliamphora hybrid i grew from seeds and now placed on multiplication media :-)
IMG_20170531_200832_917_1498122084758.jpg (56.46 KiB) Viewed 8242 times
tons of n. glabrata i subcultured a few days ago. they grow like craazy. just one out of three vessels :-)
tons of n. glabrata i subcultured a few days ago. they grow like craazy. just one out of three vessels :-)
IMG_20170618_194512_326_1498122084834.jpg (74.55 KiB) Viewed 8242 times
I hope you like the pics!

Cheers Lukas
Savior7 liked this
User avatar
By Cross
Posts:  1849
Joined:  Fri Oct 26, 2018 11:25 pm
#328466
Can I ask what mm you used? Did you make it yourself? I found some online, but if I can also just pick up stuff locally, that would be more ideal. Thanks in advance.

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