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Moderator: Matt

By David F
Location: 
Posts:  1649
Joined:  Sun Jan 02, 2011 8:41 pm
#285790
I like the look of your culture vessels.
By Doomsday
Posts:  621
Joined:  Sun Mar 07, 2010 3:11 pm
#285795
Matt wrote:Awesome! Keep us up to date.
I will! Hopefully the roots strike quickly!
David F wrote:I like the look of your culture vessels.
Thanks. These cube ones are MK-5 vessels from Caisson Labs, I like them for pictures and general viewing. They are much easier to look through than rounded glass.
By Doomsday
Posts:  621
Joined:  Sun Mar 07, 2010 3:11 pm
#285978
bijillon wrote:What is it? I know it's a callus


Happy Growing,

Dillon
Well, it was a mass of undifferentiated cells (callus) before, on hormone containing media, but since being switched to hormone-free media, the cells are being differentiated (turning into root and maybe shoot tissue) which is causing the root hair growth, and white/green/red blobs.

I'm hoping to be able to get some plants out of it within the next few months.
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By bijillon
Posts:  293
Joined:  Mon Mar 28, 2016 6:58 pm
#285992
So it a ball of root and leaf tissue together and they'll separate?


Happy Growing,

Dillon
By Doomsday
Posts:  621
Joined:  Sun Mar 07, 2010 3:11 pm
#286014
bijillon wrote:So it a ball of root and leaf tissue together and they'll separate?


Happy Growing,

Dillon
Currently, yup! I'm hoping they start to form individual plants that I can break apart, but I might have to chop it up depending on how it continues growing. I might even have to put it on more hormone to get little plant shoots to form, but it looks like that might be unnecessary, with how well it's been differentiating the newly growing tissue.

It's wild looking, isn't it?!
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By coffeenflowers
Posts:  119
Joined:  Fri Aug 05, 2016 6:06 am
#286317
Uh. So admittedly I'm not familiar with plant tissue culture but I do mammalian and insect cell culture for my job and my first thought is that the fuzzy brown stuff is contamination (aka mold).

Crystal
grow list: http://www.flytrapcare.com/phpBB3/viewtopic.php?t=32983
By Doomsday
Posts:  621
Joined:  Sun Mar 07, 2010 3:11 pm
#286364
Matt wrote:
Doomsday wrote:This Dionaea callus is starting to look more and more like an animal, every day.
That's really bizarre looking!
Isn't it?! The thing just keeps transforming. I'm curious to see what comes out of it
coffeenflowers wrote:Uh. So admittedly I'm not familiar with plant tissue culture but I do mammalian and insect cell culture for my job and my first thought is that the fuzzy brown stuff is contamination (aka mold).

Crystal
grow list: http://www.flytrapcare.com/phpBB3/viewtopic.php?t=32983
I would've thought so, expect it's not spreading to the media, and the hair's formed on the part of the callus that was originally going to be the seedlings root.
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