- Fri Aug 05, 2016 2:48 am
#270541
Hey all,
I recently built myself a glove box to do some tissue culture work in, and I have been debating on how I am going to make sure the inside is sterile once I close it up. I have been thinking of taking all of the hot sterile water and media jars, and putting them inside on the silicone mat, closing up the box with a spray bottle of a sterilizing agent inside, and then spraying it down. I'd then let the media and water cool down in the sterile glove box, and would come back to work when everything is room temperature, and possibly spray again if needed.
Which do you think would work best as a sterilizing agent for the whole inside of the box:
-Bleach 10-30%
-H2O2 ~3% (storebought)
-Isopropyl Alcohol 91%
-White Vinegar and then H2O2 (http://cleaningbusinesstoday.com/blog/h ... ecting-duo)
-Premixed PAA (using white vinegar and h2o2)
-Other
My glovebox is a plastic storage container that I modified, with some pvc pipe inserts, so the chemicals can't be able to damage any of that. If any of the above chemicals are known to damage plastics, please let me know.
I recently built myself a glove box to do some tissue culture work in, and I have been debating on how I am going to make sure the inside is sterile once I close it up. I have been thinking of taking all of the hot sterile water and media jars, and putting them inside on the silicone mat, closing up the box with a spray bottle of a sterilizing agent inside, and then spraying it down. I'd then let the media and water cool down in the sterile glove box, and would come back to work when everything is room temperature, and possibly spray again if needed.
Which do you think would work best as a sterilizing agent for the whole inside of the box:
-Bleach 10-30%
-H2O2 ~3% (storebought)
-Isopropyl Alcohol 91%
-White Vinegar and then H2O2 (http://cleaningbusinesstoday.com/blog/h ... ecting-duo)
-Premixed PAA (using white vinegar and h2o2)
-Other
My glovebox is a plastic storage container that I modified, with some pvc pipe inserts, so the chemicals can't be able to damage any of that. If any of the above chemicals are known to damage plastics, please let me know.