FlytrapCare Carnivorous Plant Forums

Sponsored by FlytrapStore.com

Discuss all micropropagation related topics here.

Moderator: Matt

By BigBella
Posts:  280
Joined:  Fri Jun 17, 2011 9:18 pm
#268238
blucksy wrote:This would be for nepenthes seeds and VFT. Maybe a cephalotus leaf also

Sent from my LG-H815 using Tapatalk
I have had successes with peracetic acid -- 6% acetic acid and hydrogen peroxide -- and dilute bleach solutions as well. There are threads on preparing the acid at home; and always choose a brand name bleach, since the concentration of sodium hydrochlorite varies a great deal by brand -- even by a given season. You always want to know a given concentration of any prepared solution.

Cephalotus leaves oxidize very easily, so I would treat those with kid gloves; so too Nepenthes seed . . .
By DrNo7
Posts:  122
Joined:  Wed Jul 22, 2015 6:35 pm
#269555
I have had great sucess using 1% silver nitrate combined with 70% ethanol through 5 minute exposures.
By Doomsday
Posts:  621
Joined:  Sun Mar 07, 2010 3:11 pm
#269674
BigBella wrote:
blucksy wrote:This would be for nepenthes seeds and VFT. Maybe a cephalotus leaf also

Sent from my LG-H815 using Tapatalk
I have had successes with peracetic acid -- 6% acetic acid and hydrogen peroxide -- and dilute bleach solutions as well. There are threads on preparing the acid at home; and always choose a brand name bleach, since the concentration of sodium hydrochlorite varies a great deal by brand -- even by a given season. You always want to know a given concentration of any prepared solution.

Cephalotus leaves oxidize very easily, so I would treat those with kid gloves; so too Nepenthes seed . . .
How do you go about using the peracetic acid to sterilize seeds (and explants if you do that too), in regards to timing and rinsing?
By BigBella
Posts:  280
Joined:  Fri Jun 17, 2011 9:18 pm
#269677
Doomsday wrote:
BigBella wrote:
blucksy wrote:This would be for nepenthes seeds and VFT. Maybe a cephalotus leaf also

Sent from my LG-H815 using Tapatalk
I have had successes with peracetic acid -- 6% acetic acid and hydrogen peroxide -- and dilute bleach solutions as well. There are threads on preparing the acid at home; and always choose a brand name bleach, since the concentration of sodium hydrochlorite varies a great deal by brand -- even by a given season. You always want to know a given concentration of any prepared solution.

Cephalotus leaves oxidize very easily, so I would treat those with kid gloves; so too Nepenthes seed . . .
How do you go about using the peracetic acid to sterilize seeds (and explants if you do that too), in regards to timing and rinsing?
The fact is -- I don't use peracetic acid any longer, not since the development of PPM. I typically soaked seeds and explants upwards of twenty minutes, followed by an H202 soak of five minutes, without rinsing . . .
BigBella liked this
By BigBella
Posts:  280
Joined:  Fri Jun 17, 2011 9:18 pm
#269723
DrNo7 wrote:I have had great sucess using 1% silver nitrate combined with 70% ethanol through 5 minute exposures.
I too have used silver nitrate in the past; and there are some silver ion-based products, such as Fungigone on the market that I have tried; though, it is a bit more difficult and pricey, for most hobbyists, to procure than supermarket white vinegar and 2% H202.

Provided that sterile technique is practiced; that seeds and tissue are treated carefully; peracetic acid can be quite effective . . .
Repotting carnivorous plants

@optique , We call it turf, I've used it before o[…]

where is a good place to buy vfts in australia. he[…]

Fishing

Hi Chef, I'm interested in whatever you have that […]

Basal shoots cutting questions

Took 5 basal shoots off the mother that were rootl[…]

Dionaea m. ‘Ginormous’

For me it grew tall leaves with small traps, but v[…]

Nep/Heli bundle

Here’s some pics of a platy vog (not this o[…]

Polimbo 52

Seedlings in the beginning of their third year. Th[…]

Seeds received. Thanks Chef and donors!

Support the community - Shop at FlytrapStore.com!