- Sun Jun 12, 2016 4:16 pm
#265089
I am Darren. I have an interest in doing tissue culture Venus flytraps 1 year ago. I gotten some germination of vft seeds here and there but they didn't grow to anything bigger than 2cm even after 5-6months.
I am using 1/2 of the Ms Media with vitamin.Here the composition of the Ms media I used before diluting to 50% strength
Major salts (macronutrients)
Ammonium nitrate (NH4NO3) 1,650 mg/l
Calcium chloride (CaCl2 · 2H2O) 440 mg/l
Magnesium sulphate (MgSO4 · 7H2O)370 mg/l
Potassium phosphate (KH2PO4) 170 mg/l
Potassium nitrate (KNO3) 1,900 mg/l
Minor salts (micronutrients)
Boric acid (H3BO3) 6.2 mg/l
Cobalt chloride (CoCl2 · 6H2O) 0.025 mg/l
Cupric sulphate (CuSO4 · 5H2O) 0.025 mg/l
Ferrous sulphate (FeSO4 · 7H2O) 27.8 mg/l
Manganese sulphate (MnSO4 · 4H2O)22.3 mg/l
Potassium iodide (KI) 0.83 mg/l
Sodium molybdate (Na2MoO4 · 2H2O)0.25 mg/l
Zinc sulphate (ZnSO4·7H2O) 8.6 mg/l
Na2EDTA · 2H2O 37.2 mg/l
Vitamins and organics
Inositol 100 mg/l
Niacin 0.5 mg/l
Pyridoxine · HCl 0.5 mg/l
Thiamine · HCl 0.1 mg/l
Glycine 2 mg/l
I basically add 4.74g into 2 litre of water and add 30g/litre of sugar and agar to make 1/2MS
I just realised that I am dividing macro and micro nutrients and vitamin by 2 .
Medium i wish to prepare 1/2 MS medium with full vitamins
But for 1/2 MS with full vitamin, does it mean that
(1/2x Macro nutrients , 1/1x micronutrient ,1/1xvitamins)
or
(1/2x Macro nutrients , 1/2 x micronutrient ,1/2 x vitamins)
Does this make any differences on the growth of the plants?I plant to use it on drosera and vft seeds.which do you think is better?
For full vitamins does it refers to this type and amount
Vitamins and organics
Inositol 100 mg/l
Niacin 0.5 mg/l
Pyridoxine · HCl 0.5 mg/l
Thiamine · HCl 0.1 mg/l
Glycine 2 mg/l
I really hope you can help me out here.Thanks a lot!
Warmest regards,
Darren
I am using 1/2 of the Ms Media with vitamin.Here the composition of the Ms media I used before diluting to 50% strength
Major salts (macronutrients)
Ammonium nitrate (NH4NO3) 1,650 mg/l
Calcium chloride (CaCl2 · 2H2O) 440 mg/l
Magnesium sulphate (MgSO4 · 7H2O)370 mg/l
Potassium phosphate (KH2PO4) 170 mg/l
Potassium nitrate (KNO3) 1,900 mg/l
Minor salts (micronutrients)
Boric acid (H3BO3) 6.2 mg/l
Cobalt chloride (CoCl2 · 6H2O) 0.025 mg/l
Cupric sulphate (CuSO4 · 5H2O) 0.025 mg/l
Ferrous sulphate (FeSO4 · 7H2O) 27.8 mg/l
Manganese sulphate (MnSO4 · 4H2O)22.3 mg/l
Potassium iodide (KI) 0.83 mg/l
Sodium molybdate (Na2MoO4 · 2H2O)0.25 mg/l
Zinc sulphate (ZnSO4·7H2O) 8.6 mg/l
Na2EDTA · 2H2O 37.2 mg/l
Vitamins and organics
Inositol 100 mg/l
Niacin 0.5 mg/l
Pyridoxine · HCl 0.5 mg/l
Thiamine · HCl 0.1 mg/l
Glycine 2 mg/l
I basically add 4.74g into 2 litre of water and add 30g/litre of sugar and agar to make 1/2MS
I just realised that I am dividing macro and micro nutrients and vitamin by 2 .
Medium i wish to prepare 1/2 MS medium with full vitamins
But for 1/2 MS with full vitamin, does it mean that
(1/2x Macro nutrients , 1/1x micronutrient ,1/1xvitamins)
or
(1/2x Macro nutrients , 1/2 x micronutrient ,1/2 x vitamins)
Does this make any differences on the growth of the plants?I plant to use it on drosera and vft seeds.which do you think is better?
For full vitamins does it refers to this type and amount
Vitamins and organics
Inositol 100 mg/l
Niacin 0.5 mg/l
Pyridoxine · HCl 0.5 mg/l
Thiamine · HCl 0.1 mg/l
Glycine 2 mg/l
I really hope you can help me out here.Thanks a lot!
Warmest regards,
Darren