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By drosophyllum
Posts:  32
Joined:  Sun Oct 31, 2010 12:11 pm
#109151
Yes, it's very early to say if it works o not, but keep us informed.

I will receive NaDCC in the next weeks, during summer I will test with many vft I have in the greenhouse.
By stry67
Posts:  99
Joined:  Mon May 02, 2011 6:54 pm
#109256
Matt wrote:I picked up some ClearView granular Chlorinating Concentrate 56 which has an active ingredient of 99% Sodium dichloro-s-triazinetrione dihydrate.

Is that the same concentration and chemical you're working with stry67?
It's the exact same chemical composition but the brand I have Leisure Time Spa 56. I paid 15 bucks for 2lbs and that including shipping. Just for info, Phytotech sells NaDCC at 1 kilogram (same chemical comp) at $82 with a $25 Hazmat fee and a shipping charge. 1 Kg is like 2.2 lbs I think. No brainer there.
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By Matt
Location: 
Posts:  22523
Joined:  Mon Apr 21, 2008 11:28 pm
#109298
Right, I was kind of following the NaDCC thread on the hometissueculture list serve. I almost ordered the one you did from Amazon, but I decided to support the local pool store instead. Cost was $16 for 2 pounds and I didn't have to wait for it to ship :)
By Savior7
Posts:  29
Joined:  Mon Jul 27, 2015 9:10 am
#251162
Has anybody got an good sterilization protocoll for VFT with NaDCC ? 12h+ if possible.
Greetings
By cvlover
Posts:  447
Joined:  Thu Apr 23, 2009 4:59 pm
#251173
Read the fist page of threat from BradR:

=======
.....
All of the above cultures except for the 'Fang' culture were sterilized using NaDCC.

Abstract
..... Solutions of Sodium dichloroisocyanurate were more effective at high concentrations (5000 ppm) than a commercially available bleach for disinfection of shoot cultures. Sodium dichloroisocyanurate was also used at low concentrations (300 ppm) for longer periods (24 h-48 h) to disinfect shoot explants from the field, and was at least as effective for sterilisation as a combination of Mercuric Chloride and Calcium hypochlorite.
======

NaDCC is the excellent disinfectant. It's cheap, easy to use store and prepare, available at Walmart and other hardware stores, less toxic to explant and environment than Clorox, Calcium Hypochloride, Mercuric Chloride . etc... Have been using it for orchid & CPs seeds and explants. 300 - 500 ppm for 24 hours is a good way to sterilize sensitive explants.
By Mufasa
Posts:  858
Joined:  Sun Feb 23, 2014 4:45 am
#251233
cvlover wrote:Read the fist page of threat from BradR:

=======
.....
All of the above cultures except for the 'Fang' culture were sterilized using NaDCC.

Abstract
..... Solutions of Sodium dichloroisocyanurate were more effective at high concentrations (5000 ppm) than a commercially available bleach for disinfection of shoot cultures. Sodium dichloroisocyanurate was also used at low concentrations (300 ppm) for longer periods (24 h-48 h) to disinfect shoot explants from the field, and was at least as effective for sterilisation as a combination of Mercuric Chloride and Calcium hypochlorite.
======

NaDCC is the excellent disinfectant. It's cheap, easy to use store and prepare, available at Walmart and other hardware stores, less toxic to explant and environment than Clorox, Calcium Hypochloride, Mercuric Chloride . etc... Have been using it for orchid & CPs seeds and explants. 300 - 500 ppm for 24 hours is a good way to sterilize sensitive explants.
NaDCC is hydrogen peroxide, right?

how do those PPM ratings translate into percentages?
By Savior7
Posts:  29
Joined:  Mon Jul 27, 2015 9:10 am
#251257
Hello,

I think i will give it a try tomorrow and see how good it works on small VFT Explants, i will go with 400ppm and 24 hours, rinse 3x times in destilled sterile water and cut the ends off. hope it will work quite nice

best greetings
By Savior7
Posts:  29
Joined:  Mon Jul 27, 2015 9:10 am
#251600
Hey everybody,

It worked quite ok. I have to find the way that it's works better for me. Smaller explants died after 24 hours. The bigger explants were at some spots brown but still usable. I also did a try with about 350ppm but i got the same problem. I used also 2 drops of tween 20.

Has anybody got some tips for me ?

Greetings Lukas
By Mufasa
Posts:  858
Joined:  Sun Feb 23, 2014 4:45 am
#251627
you know... i actually think i recall someone mentioning they used to buy a clothes washing product... powder with nadcc in it, and soak material in it overnight
By Savior7
Posts:  29
Joined:  Mon Jul 27, 2015 9:10 am
#251655
theres an amazon link on the previous page to this stuff: http://www.amazon.com/Leisure-22337-Chl ... 524&sr=8-1

i think most use this. I got mine from a plant tissue culture shop

http://www.floralab.eu/index.php?route= ... duct_id=68

by soaking it over night you think of an time from 8-12 hours right ? did you tried it by yourself ?

greetings Lukas
By Mufasa
Posts:  858
Joined:  Sun Feb 23, 2014 4:45 am
#251661
Savior7 wrote:theres an amazon link on the previous page to this stuff: http://www.amazon.com/Leisure-22337-Chl ... 524&sr=8-1

i think most use this. I got mine from a plant tissue culture shop

http://www.floralab.eu/index.php?route= ... duct_id=68

by soaking it over night you think of an time from 8-12 hours right ? did you tried it by yourself ?

greetings Lukas
no idea.. here's the post i was talking about, make of it what you will..
http://www.flytrapcare.com/phpBB3/post1 ... an#p166894
By Savior7
Posts:  29
Joined:  Mon Jul 27, 2015 9:10 am
#251666
Just an update to the try with 400ppm NaDCC for 24 hours tissue is now 4 days old...i can see bacterial contamination or complete brown tissue... i have no idea why...
Attachments:
Dead tissue
Dead tissue
tmp_15521-20151130_194652-1097123887.jpg (183.38 KiB) Viewed 7545 times
Contamination
Contamination
tmp_15521-20151130_194634-2022280327.jpg (215.06 KiB) Viewed 7545 times
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By Matt
Location: 
Posts:  22523
Joined:  Mon Apr 21, 2008 11:28 pm
#251678
Savior7 wrote:Just an update to the try with 400ppm NaDCC for 24 hours tissue is now 4 days old...i can see bacterial contamination or complete brown tissue... i have no idea why...
That's the same results I had with my NaDCC experiments. Very little success. Either dead tissue or contamination. I gave up on that method and went back to 10% sodium hypochlorite (bleach).
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