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Moderator: Matt

By Savior7
Posts:  29
Joined:  Mon Jul 27, 2015 9:10 am
#237972
Hey guys,

I start to really get into TC. I made some media which worked nice without contamination. so i wanted to know if my sterilization protocol would work for Explants seeds.

seeds i got:
D. Rotundifolia
D. Burmanii
D. Muscipulia
D. Capensis

Explants:

some VFT

-wash in destillted water with/without antibacterial soap 2-3 min (skip for seed)
-quick dip in 70-90% isopropyl (skip for seed)
-let soak in 3% H2O2
seeds: 3 min
explant 15 min
-rinse 2 times in sterilized, distilled water

would this work ?

and do i need Clorox bleach ?

greetings
Lukas
Dionaea m. ‘Ginormous’

Could you send photos?

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