- Tue Oct 14, 2014 11:16 am
#214470
Hello,
I want o ask you what is your contamination rate during subcultures?
I lose almost one third of my vessels after every subculture. I don't have laminar flow hood. I use only big fish tank. Inside of my aquarium, I have bottle of isopropyl alcohol for my foreceps and scalpel and I always spray everything inside box with isopropyl alcohol. I cut plants on sterilized petri dish (steam sterilized in preassure cooker). Is it normal to suffer such dramatic losses? Contamination rates in good laboratories are under 4 % according to literature. What percentage did you achieve witout LFH?
I want o ask you what is your contamination rate during subcultures?
I lose almost one third of my vessels after every subculture. I don't have laminar flow hood. I use only big fish tank. Inside of my aquarium, I have bottle of isopropyl alcohol for my foreceps and scalpel and I always spray everything inside box with isopropyl alcohol. I cut plants on sterilized petri dish (steam sterilized in preassure cooker). Is it normal to suffer such dramatic losses? Contamination rates in good laboratories are under 4 % according to literature. What percentage did you achieve witout LFH?