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Anyone have a way to ID bacteria or fungus in TC?

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Moderator: Matt

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Re: Anyone have a way to ID bacteria or fungus in TC?

By Aoli wang
Posts:  115
Joined:  Wed Sep 25, 2013 9:11 am
-  Thu Oct 23, 2014 2:44 am #215113
If you have a large budget,it will be fairly easy to determine which microbe contamed your culture.

we can apply CTAB or someother methods to extract whole DNA from pure culture,then amplify some conserved regions of its gene,sequence them and subject to database(NCBI has such a database for free i remenber)and download related sequences to build a Evolutionary tree.from the tree we can locate which genus or spieces it belongs to.
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